Mouse Insulin Elisa Alpco

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Male and female mice from the HMDP were obtained from the Jackson Laboratories and bred at UCLA. At 8 to 10 weeks of age mice were switched from a chow diet to a high fat diet for 8 weeks. After 8 weeks on high fat diet mice were fasted for 4 hours (beginning at 6 am) and processed for plasma and tissues (as described below). High fat diet was obtained from Research Diets, Inc. (#D12266B) and contains 31.8 % Kcal from fat. 3. Body composition, NMR (B. Parks) NMR (Bruker) scanning of live mice to determine fat and lean mass was performed at 0, 2, 4, 6, and 8 weeks after the start of high fat diet. 4. Plasma lipids, insulin & glucose assays (L. Castellani) Following 4 hour fast mice were bled retro-orbitally under isoflurane into EDTA-coated microtubes and Heparin-coated microtubes and kept on ice. Blood was centrifuged for 10 minutes at 12,000 RPM and the plasma was collected and stored at -80C. General Overview: The assays that we routinely perform are total cholesterol, HDL cholesterol, unesterified cholesterol (UC), unesterified (free) fatty acids (FFA), triglycerides (TG) and glucose. Total plasma cholesterol is assayed after treating the samples with cholesterol esterase to hydrolyze cholesterol esters and then performing the cholesterol assay, which will now encompass all of the cholesterol that was in both the cholesterol ester and unesterfied cholesterol pools. Hence, this is the total cholesterol assay. Unesterified cholesterol is assayed by performing the cholesterol assay without treating the plasma sample with cholesterol esterase, so that only the free or unesterified cholesterol is determined. This value can then be subtracted from the total cholesterol to calculate the esterfied or cholesterol esters (CE). The HDL cholesterol is determined by performing t Continue reading >>

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Learn about our STELLUX line of chemiluninescent assays. Quantify levels in plasma and urine with ready-to-use controls. Quantify levels in plasma and urine with ready-to-use controls. Measure IGF in mouse, rat or human serum and plasma. Sensitive, wide range, low sample ELISAs for Research Use Only. Quantify levels in plasma, serum, urine and stool in 2 hours. Learn about our STELLUX line of chemiluninescent assays. Sensitive, wide range, low sample ELISAs for Research Use Only. Quantitatively determine oxidized LDL with 10 uL of plasma or serum. Measure HMW/Total ADP in mouse serum and plasma with less sample. Quantitatively determine oxidized LDL with 10 uL of plasma or serum. Human GLP-1 ELISA - Measure low levels of GLP-1 Quantify levels in plasma and urine with ready-to-use controls. For the quantitative determination of bile acids in stool samples. Quantitatively determine oxidized LDL with 10 uL of plasma or serum. Measure HMW/Total ADP in mouse serum and plasma with less sample. Measure levels with sensitive, low sample volume ELISAs. Measure HMW and/or Total ADP in serum and plasma samples. Quantify levels in plasma and urine with ready-to-use controls. Measure IGF in mouse, rat or human serum and plasma. Ultrasensitive high range ELISA delivers quick results. Human GLP-1 ELISA - Measure low levels of GLP-1 - alpco.com Continue reading >>

Elisa Kits | Thermo Fisher Scientific

More than 1,000 protein-specific ELISA kits Ready-to-use Invitrogen ELISA kits are optimized for convenient and sensitive quantitation of a wide range of targets in various sample types, including human, mouse, rat IgG, cytokines, phosphorylated proteins, beta amyloids, extracellular and intracellular targets. Pre-coated and coat-it-yourself sandwich ELISA kits include capture and detection matched antibodies, standards, buffers, diluents, and detection reagents. Specialty ELISA formats and configurations are also available. We offer several varieties each of coated and uncoated ELISA kits, including those based on Invitrogen Instant ELISA Technology , validated for particular levels of sensitivity, formatted as sandwich and competitive ELISAs, or optimized for a various sample types. Our portfolio of ELISA kits now includes products previously sold under the Affymetrix eBioscience brand, now part of Thermo Fisher Scientific. The new, combined Invitrogen ELISA kit product family encompasses a greater range of available analytes, species, and formats. Especially noteworthy additions are the Instant ELISA kits and expanded line of immunoglobulin and isotyping kits . These kits include antibodies, standards, detection reagents, and 96-well plates that are precoated with the capture antibody. These kits contain matched antibody pairs, standards and detection reagents but require users to coat the capture antibody themselves. Develop, test, and validate your own direct or sandwich ELISA by selecting your own antibodies, reagents, plates, and other assay components. Continue reading >>

Headlines from the 2017 ADA Scientific Sessions

Gestational Exposure To Metformin Programs Improved Glucose Tolerance And Insulin Secretion In Adult Male Mouse Offspring

Gestational exposure to metformin programs improved glucose tolerance and insulin secretion in adult male mouse offspring The details of the in utero metformin exposure model have been published previously 8 . Briefly, we purchased 8 week-old virgin C57Bl6 animals from Jackson Laboratories and adapted to control diet (D02041001B, Research Diets Inc., New Brunswick, NJ, USA) for 3 weeks. Upon vaginal plug detection females were given unadulterated water or sterile water with metformin (Sigma-Aldrich, St Louis, MO) at 5 mg/mL. Water was changed weekly and the treatment was continued until birth. At that point mothers resumed the filtered water supply in the animal room. All the studies were approved by the University of Michigan Institutional Care and Use Committee and performed in accordance with their guidelines under an approved animal protocol. We harvested adult pancreata at 3 months of age from C57Bl6 metformin and control animals. We then fixed pancreata in 3.7% formalin in PBS overnight. A collagenase digestion technique was used to isolate pancreatic islets 12 . The common bile duct was perfused with 1 mg/ml collagenase XI (Sigma-Aldrich, St. Louis, MO) in HBSS (Life Technologies, Carlsbad, CA). Pancreata were digested at 37 C for 15 min, and then cold HBSS with 2.5% FBS (Life Technologies) was added. After centrifugation at 1500 rpm for 2 min, the pellet was washed 3 times with HBSS with 2.5% FBS. A histopaque gradient was then set up and pancreata were centrifuged at 2000 rpm at 10 degrees Celsius for 20 minutes. The interphase layer was collected, filtered through a 70 m cell strainer (BD Falcon, BD Biosciences, San Diego, CA) and rinsed with HBSS. The islets were plated in RPMI 1640 with 5 mM Glucose, 10% FBS, 100 IU/ml penicillin, 100 g/ml streptomycin. The Continue reading >>

Mouse Ultrasensitive Insulin Elisa From Alpco

(10) Monitoring C-Peptide Storage and Secretion in Islet -Cells In Vitro and In Vivo DiabetesDecember 8, 2015Shuaishuai Zhu, Dennis Larkin, Shusheng Lu, Candice Inouye, Leena Haataja, Arfah Anjum, Robert Kennedy, David Castle, Peter Arvan (11) PPP2R5C Couples Hepatic Glucose and Lipid Homeostasis PLoS GeneticsOctober 6, 2015Yong-Sheng Cheng, Oksana Seibert, Nora Klting, Arne Dietrich, Katrin Straburger, Sonia Fernndez-Veledo, Joan J. Vendrell, Antonio Zorzano, Matthias Blher, Stephan Herzig, Mauricio Berriel Diaz, Aurelio A. Teleman (13) Maternal protein restriction induces early-onset glucoseintolerance and alters hepatic genes expression in the peroxisome proliferator-activated receptor pathway in offspring Journal of Diabetes InvestigationDecember 11, 2014Jia Zheng, Xinhua Xiao, Qian Zhang, Miao Yu, Jianping Xu, Zhixin Wang (14) Rapamycin-mediated lifespan increase in mice is dose and sex dependent and metabolically distinct from dietary restriction. Aging cellJune 1, 2014Richard A Miller, David E Harrison, Clinton M Astle, Elizabeth Fernandez, Kevin Flurkey, Melissa Han, Martin A Javors, Xinna Li, Nancy L Nadon, James F Nelson, Scott Pletcher, Adam B Salmon, Zelton Dave Sharp, Sabrina Van Roekel, Lynn Winkleman, Randy Strong (15) Loss of Insulin Receptor in Osteoprogenitor Cells Impairs Structural Strength of Bone Journal of Diabetes ResearchMay 18, 2014Kathryn Thrailkill, R. Clay Bunn, Charles Lumpkin, Elizabeth Wahl, Gael Cockrell, Lindsey Morris, C. Ronald Kahn, John Fowlkes, Jeffry S. Nyman (16) Maternal high-fat diet modulates hepatic glucose, lipid homeostasis and gene expression in the PPAR pathway in the early life of offspring. International journal of molecular sciencesJanuary 1, 2014Jia Zheng, Xinhua Xiao, Qian Zhang, Miao Yu, Jianping Xu, Zhixin Wang Continue reading >>

Supplement To Wnt Signaling Regulates Pancreatic Cell Proliferation | Pnas

Fig. 8. Immunohistology and insulin studies of islets from control and RIP-Cre b-active mice. (A and B) Localization of b-catenin in islets from control (A) and RIP-Cre b catactive (B) mice by immunohistochemistry. b-catenin (green), DAPI (blue) and insulin (red) were detected by immunofluorescence microscopy. White arrowheads in B highlight nuclei with prominent nuclear b-catenin signal. (C-F) Pancreatic insulin levels and insulin secretion of islets from RIP-Cre bcatactive and control mice. Insulin levels were calculated on a per islet basis (C) as well as per islet DNA content (D) to normalize cell number between islets. Assessment of insulin secretion in response to step increase of arginine (E) or KCl (F) at the indicated concentrations. Fig. 9. Phenotypes after axin induction in Pdx1-tTA/TRE-Axin mice. (A) Western immunoblot to detect Myc-labeled Axin (black arrowhead) in total protein isolated from P6 mice of the indicated genotypes. (B and C) Immunofluorescent detection of BrdU incorporation in Nkx6.1+ cells in WT (B) and Pdx1-tTA/TRE-Axin (C) E16.5 embryos. (D and E) The relative number Nkx6.1+ (D), and Nkx6.1+BrdU+ (E) cell nuclei per pancreas in E16.5 WT and Pdx1-tTA/TRE-Axin embryos or in Pdx1-tTA/TRE-Axin embryos on Doxycycline from the time of conception. Data are presented as the average SEM. P values are indicated in the figures. Data are from at least five mice per genotype. (Original magnification: 100.) Fig. 10. i.p. glucose challenge of P28 mice after a 16-h overnight fast. Black line and open circle, Pdx1-tTA/TRE-Axin mice; red line, Pdx1-tTA littermates; blue line, TRE-Axin littermates; black line and closed circle, WT mice. Indicated P values are for data from Pdx1-tTA/TRE-Axin and Pdx1-tTA mice. All data are from at least three litters, with fou Continue reading >>

Lipid Nanoparticle Delivery Of Glucagon Receptor Sirna Improves Glucose Homeostasis In Mouse Models Of Diabetes

This is an open access article under the CC BY-NC-ND license (Hyperglucagonemia is present in many forms of diabetes and contributes to hyperglycemia, and glucagon suppression can ameliorate diabetes in mice. Leptin, a glucagon suppressor, can also reverse diabetes in rodents. Lipid nanoparticle (LNP) delivery of small interfering RNA (siRNA) effectively targets the liver and is in clinical trials for the treatment of various diseases. We compared the effectiveness of glucagon receptor (Gcgr)-siRNA delivered via LNPs to leptin in two mouse models of diabetes. Gcgr siRNA encapsulated into LNPs or leptin was administered to mice with diabetes due to injection of the -cell toxin streptozotocin (STZ) alone or combined with high fat diet (HFD/STZ). In STZ-diabetic mice, a single injection of Gcgr siRNA lowered blood glucose levels for 3 weeks, improved glucose tolerance, and normalized plasma ketones levels, while leptin therapy normalized blood glucose levels, oral glucose tolerance, and plasma ketones, and suppressed lipid metabolism. In contrast, in HFD/STZ-diabetic mice, Gcgr siRNA lowered blood glucose levels for 2 months, improved oral glucose tolerance, and reduced HbA1c, while leptin had no beneficial effects. While leptin may be more effective than Gcgr siRNA at normalizing both glucose and lipid metabolism in STZ diabetes, Gcgr siRNA is more effective at reducing blood glucose levels in HFD/STZ diabetes. Keywords: Type 1 diabetes, Type 2 diabetes, Glucose metabolism, Lipid metabolism, Glucagon, Leptin Abbreviations: AUC, area under the curve; FVII, factor VII; Gcgr, glucagon receptor; HFD, high fat diet; KO, knockout; LFD, low fat diet; LNP, lipid nanoparticle; siRNA, small interfering RNA; STZ, streptozotocin; WT, wildtype Hyperglucagonemia is present in type 1 [ Continue reading >>

Mk-0626, A Selective Dpp-4 Inhibitor, Attenuates Hepatic Steatosis In Ob/ob Mice

MK-0626, a selective DPP-4 inhibitor, attenuates hepatic steatosis in ob/ob mice Number of Hits and Downloads for This Article Nov 21, 2014 (publication date) through Apr 26, 2018 Baishideng Publishing Group Inc, 7901 Stoneridge Drive, Suite 501, Pleasanton, CA 94588, USA Copyright 2014 Baishideng Publishing Group Inc. All rights reserved. World J Gastroenterol.Nov 21, 2014;20(43): 16227-16235 Published online Nov 21, 2014.doi: 10.3748/wjg.v20.i43.16227 MK-0626, a selective DPP-4 inhibitor, attenuates hepatic steatosis in ob/ob mice Tatsuya Ohyama, Ken Sato, Yuichi Yamazaki, Hiroaki Hashizume, Norio Horiguchi, Satoru Kakizaki, Masatomo Mori, Motoyasu Kusano, Masanobu Yamada Tatsuya Ohyama, Ken Sato, Yuichi Yamazaki, Hiroaki Hashizume, Norio Horiguchi, Satoru Kakizaki, Masatomo Mori, Masanobu Yamada, Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan Motoyasu Kusano, Department of Endoscopy and Endoscopic Surgery, Gunma University Hospital, Gunma 371-8511, Japan Author contributions: Sato K designed the research, wrote the paper, and analyzed the data; Ohyama T and Yamazaki Y performed the experiment; Hashizume H, Horiguchi N and Kakizaki S analyzed the data; Mori M and Kusano M revised the draft critically for important intellectual content; Yamada M gave final approval of the version to be published. Correspondence to: Ken Sato, MD, PhD, Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. AIM: To investigate the mechanism and in vivo effects of MK-0626, a dipeptidyl peptidase-4 inhibitor, on hepatic steatosis using ob/ob mice. METHODS: We analyzed obese (ob/ob) 8-wk-old male mice that had been randomly divided int Continue reading >>

Mouse Insulin Elisa From Alpco

(1) Repletion of branched chain amino acids reverses mTORC1 signaling but not improved metabolism during dietary protein dilution Molecular MetabolismJune 24, 2017Adriano Maida, Jessica S.K. Chan, Kim A. Sjberg, Annika Zota, Dieter Schmoll, Bente Kiens, Stephan Herzig, Adam J. Rose (2) CD47 blocking antibodies restore phagocytosis and prevent atherosclerosis NatureFebruary 4, 2017Yoko Kojima, Jens-Peter Volkmer, Kelly McKenna, Mete Civelek, A. Jake Lusis, Clint Miller, Daniel Direnzo, Vivek Nanda, Jianqin Ye, Andrew Connolly, Eric Schadt, Thomas Quertermous, Paola Betancur, Lars Maegdefessel, Ljubica Perisic, Ulf Hedin, Irv Weissman, Nicholas J. Leeper (4) Opposing impacts on healthspan and longevity by limiting dietary selenium in telomere dysfunctional mice Aging CellSeptember 21, 2016Ryan T. Wu, Lei Cao, Elliot Mattson, Kenneth W. Witwer, Jay Cao, Huawei Zeng, Xin He, Gerald F. Combs, Wen-Hsing Cheng (5) Hepatic expression of cytochrome P450 in Zucker diabetic fatty rats Food and Chemical ToxicologyAugust 5, 2016So Young Park, Chung Hyeon Kim, Ji Yoon Lee, Jang Su Jeon, Min Ju Kim, Song Hee Chae, Hyoung Chin Kim, Soo Jin Oh, Sang Kyum Kim Continue reading >>

Alpco Launches Their First Stellux Chemiluminescent Elisa

ALPCO Launches Their First STELLUX Chemiluminescent ELISA ALPCO announces the launch of their STELLUX Rodent Insulin ELISA, the first ELISA launched in their new STELLUX Chemiluminescent assay product line. Salem, NH, April 26, 2013 --( PR.com )-- For over twenty years, ALPCO has been a provider of immunoassay kits and other life science solutions to researchers in the academic, pre-clinical and clinical communities. ALPCO will begin offering their STELLUX Rodent Insulin ELISA on April 25, 2013, the first ELISA launched in their new STELLUX Chemiluminescent assay product line. Many commercial mouse and rat insulin ELISAs require several dilution steps to ensure concentrations fall within a limited standard curve range, resulting in increased sample preparation time and added labor costs. Chemiluminescent assays provide rapid and sensitive detection while exhibiting wide dynamic ranges. ALPCO has developed the STELLUX Rodent Insulin ELISA to enable researchers to confidently measure insulin in both rats and mice from 0.1 to 150 ng/mL, using just 5 mL of neat serum or plasma. This new Rodent Insulin Chemiluminescent ELISA is unparalleled in the industry. It allows our customers accurate results with only a 5 L sample size, says Martin Blankfard, Vice President of Scientific Operations, Our chemiluminescent detection systems allow for a dynamic range that suits both live rodent models as well as in vitro islet studies. This is a problem weve been asked to solve time and time again, and this system provides the answer. My goal is that all the assays in our new STELLUX Chemiluminescent product portfolio prove to set new standards in the field. This is what our customers have come to expect from us, and what we aim to deliver. ALPCOs President, Sean Conley added the followin Continue reading >>

Insulin Protocol

Protocol for: ELISA insulin protocol Prepared by: Rong Yuan 0.0 Abstract: This test determines the amount of insulin in mouse plasma in units of ng/ml. Insulin levels are determined using plasma from blood. Blood is collected using 7.5ul Sodium Heparin 1000 units with Heparin coated capillary tubes. ELISA is a solid phase two-site enzyme immunoassay. 1.0 Experiment layout: This test uses a Spectra Max 190 ELISA plate reader using Softmax software driven by a PC. 2.0 Supplied materials and materials needed: 2.1 Supplied materials: The ELISA insulin kit is purchased from ALPCO Diagnostics, P.O. Box 451, Windham, NH 03087. Kit contains: (1) Microplates (twelve strips, eight wells each coated with mouse anti-insulin) (2) Standards of 0.025, 0.063, 0.25, 0.55, 1.40, 4.0, 7.5 ng/ml which are lyophilized and need to be reconstituted with 1ml of redistilled water. (3) Zero Standard, which is already reconstituted. (4) Anti-Insulin Conjugate Stock solution is prepared by diluting 50ul Conjugate Stock Solution with 500ul Conjugate Buffer for each strip. (5) Washing solution is prepared by diluting 40ml of Washing Solution Concentrate into 800ml of redistilled water. (6) TMB Substrate and Stop solution are already prepared in kit. 2.2 Materials needed: (1) 5, 25, 50 ul micropipette, 50 and 250 repeating pipettes (2) containers for reagent preparation and Mammalian/Mouse Insulin Two level control (3) microplate rotator (4) redistilled water. 3.0 Setup: Make a template as to where your Blank, Standards, Controls and Samples are going to be placed within the microplate. Depending whether single, duplicate, or triplicate samples are run, the plate should be set up this way: 1 2 3 4 5 A STANDARD STANDARD SAMPLE SAMPLE SAMPLE B STANDARD STANDARD SAMPLE SAMPLE SAMPLE C STANDARD STANDARD Continue reading >>

Pancreatic -cell Research Associated With Metabolic Syndrome And Type 2 Diabetes

Pancreatic -Cell research associated with metabolic syndrome and type 2 diabetes Metabolic syndrome (MetS) encompasses multiple risk determinants including obesity, hypertension, lipid abnormalities, and insulin resistance.1 Currently, there is no clear answer as to which factor, if any, is the original cause of MetS and which are consequential to the syndrome.2 Even with this uncertainty, these risk determinants play an important role in type 2 diabetes, as evidenced by the fact that 65% to 85% of individuals with type 2 diabetes also have metabolic syndrome.3,4,5 One area of research that overlaps MetS and type 2 diabetes focuses on how the pancreatic b-cell combats hyperglycemia caused by insulin resistant peripheral tissue. For MetS, pancreatic b-cells secrete more insulin to compensate for insulin resistance. Similarly, the impaired glucose tolerance observed in patients with pre-type 2 diabetes is also compensated for by increased secretion of insulin by pancreatic b-cells.6 As type 2 diabetes progresses, however, the ability of pancreatic b-cells to secrete sufficient levels of insulin to stimulate glucose uptake by the insulin resistant peripheral tissue diminishes due to decreased pancreatic b-cell mass/function, and, as a result, blood glucose levels rise.7 The above described alterations in b-cell secretion are not limited to just insulin and C-peptide. Greater secretion of proinsulin is also attributed to MetS- and type 2 diabetes-associated b-cell dysfunction.8,9 For example, as glucose intolerance progresses to type 2 diabetes, the release of proinsulin can surpass that of insulin as a result of incomplete or no post-translational processing of proinsulin,10 and can be observed as an increase in the proinsulin:insulin ratio.11 Much of the above discussion Continue reading >>

Increased Yield And Improved Transplantation Outcome Of Mouse Islets With Bovine Serum Albumin

Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin 1Division of Immunogenetics, Department of Pediatrics, John G. Rangos Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA 2Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA Received 26 September 2012; Accepted 11 November 2012 Copyright 2012 Suzanne Bertera et al. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Isolation and transplantation of rodent islets are frequently used as a tool for predicting the behavior of new protocols for islet allotransplants in type 1 diabetes patients. Bovine serum albumin (BSA) is recognized as a protease inhibitor possibly protecting function and viability in islets. For this study, the addition of 0.2% BSA to the isolation protocol resulted in a 30% increase in islet yields while other parameters, such as viability and function, retained high islet quality. In vivo, a minimal mass of 70 BSA treated islets showed their ability to control glycemia levels in diabetic mice by bringing the average blood glucose to mg/dL without BSA. Our results show that the simple addition of BSA to the isolation protocol constitutes a reliable and reproducible method for increasing islet yield. Also adding BSA to the transplantation medium improves islet function in vivo. The method outlined here can reduce the overall number of animals needed per experiment and also reduce the time and resources needed for islet preparation. With the increased interest in finding a cure for type 1 diabetes, basic res Continue reading >>

Mercodia Ultrasensitive Mouse Insulin Elisa

This site uses cookies. By continuing to browse the site, you are agreeing to our use of cookies. Find out more Mercodia Ultrasensitive Mouse Insulin ELISA The Mercodia Ultrasensitive Mouse Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin. High sensitivity - Assay range 0.025 - 1.5g/L Mercodia Ultrasensitive Mouse Insulin ELISA provides a method for the quantitative determination of insulin in mouse serum, plasma or cell culture medium. Mercodia Ultrasensitive Mouse Insulin ELISA If you register and login product prices are shown in the list above. Without a user account it is still possible to request a quote by sending us your cart. Samples: Serum, plasma, cell culture medium The detection limit is 0.025 g/L as determined with the methodology described in ISO11843-Part 4. Recovery upon addition is 76 - 94 % (85 %). Recovery upon dilution is 110 - 132 % (123 %). Each sample was analyzed in 4 replicates on 16 different Serum, plasma, cell culture medium. Sample volume 25L. Read more about the 10L alternative protocol. Grossly lipemic, icteric or haemolyzed samples do not interfere Mercodia Mouse Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the sample reacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution. Add 25 L Calibrators, controls and samples Shake for approximately 5 seconds on shaker Continue reading >>

Plasma Protein Biomarkers Correlated With The Development Of Diet-induced Type 2 Diabetes In Mice

Plasma Protein Biomarkers Correlated with the Development of Diet-Induced Type 2 Diabetes in Mice Shigeru Okada , Edward O. List , Sudha Sankaran , and John J. Kopchick Edison Biotechnology Institute, Konneker Research Laboratories, Ohio University, The Ridges, Bldg. 25, Athens, OH 45701-2979, USA, Department of Pediatrics, College of Osteopathic Medicine, Ohio University, Athens, OH, USA Edison Biotechnology Institute, Konneker Research Laboratories, Ohio University, The Ridges, Bldg. 25, Athens, OH 45701-2979, USA, Department of Biomedical Science, College of Osteopathic Medicine, Ohio University, Athens, OH, USA Shigeru Okada, Edison Biotechnology Institute, Konneker Research Laboratories, Ohio University, The Ridges, Bldg. 25, Athens, OH 45701-2979, USA, Department of Pediatrics, College of Osteopathic Medicine, Ohio University, Athens, OH, USA; See other articles in PMC that cite the published article. Early detection, assessment of disease progression, and application of an appropriate therapeutic intervention are all important for the care of patients with type 2 diabetes. Currently, however, there is no simple test for early detection of type 2 diabetes. Established diagnostic tests for the disease including oral glucose tolerance, fasting blood glucose, and hemoglobin A1c are relatively late markers where the disease has already progressed. Since blood is in direct contact with many tissues, we hypothesized that pathological tissue changes are likely to be reflected in proteomic profiles of plasma. Mice were reared either on regular chow or a high-fat diet at weaning and several physiological responses (i.e., weight, fasting plasma glucose and insulin, and glucose tolerance) were monitored at regular time intervals. Plasma was collected at regular intervals fo Continue reading >>