Insulin Injection And Hemolymph Extraction To Measure Insulin Sensitivity In Adult Drosophila Melanogaster
Protocol 1. Insulin Solution Preparation Prepare fresh bovine insulin solution by dissolving insulin in PBS to achieve the concentration of 0.01 mg/ml. Both insulin/PBS and control PBS solutions must be kept on ice throughout the injection procedure. These solutions should be prepared with 0.5% (v/v) FD&C Blue no. 1 food coloring. 2. Needle Preparation and Injection Set-up Prepare capillary glass needles using a micropipette puller.The following puller settings produce needles of sufficient quality for injection: Heat, 345; Pull, 210; Velocity, 100; Time, 200 (100ms). Freshly pulled needles must be blunted by pressing the tip through a Kimwipe tissue. This blunting process removes the elongated high resistance tip and produces a stouter tip with a larger pore diameter. Place blunted needles tip-up in a microcentrifuge tube containing the insulin/PBS solution or PBS alone. Capillary action results in back-filling of each needle. Observe the tip of the needle under a stereomicroscope to ensure that no debris or air bubbles appear at the tip. Discard any needles that do not fill cleanly. Insert filled needles into the manual microinjector needle holder and position the needle holder with a micromanipulator so that the needle tip is visible through a stereomicroscope. Apply positive pressure on the fluid column in the needle by turning the manual microinjector micrometer knob attached to the plunger on the microinjector syringe. Verify that sufficient pressure for fluid displacement has been applied by touching a Kimwipe to the tip of the needle and confirming fluid flow. Once the needle has been prepared for injection, affix a calibration chart to the needle shaft with clear tape, and bring the needle tip into focus through a stereomicroscope under 20X magnification. 3. Fl Continue reading >>
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Insulin Injection Sites: Where And How To Inject
Insulin is a hormone that helps cells use glucose (sugar) for energy. It works as a “key,” allowing the sugar to go from the blood and into the cell. In type 1 diabetes, the body doesn’t make insulin. In type 2 diabetes, the body doesn’t use insulin correctly, which can lead to the pancreas not being able to produce enough — or any, depending on the progression of the disease —insulin to meet your body’s needs. Diabetes is normally managed with diet and exercise, with medications, including insulin, added as needed. If you have type 1 diabetes, insulin is required for life. This may seem difficult at first, but you can learn to successfully administer insulin with the support of your healthcare team, determination, and a little practice. There are different ways to take insulin, including syringes, insulin pens, insulin pumps, and jet injectors. Your doctor will help you decide which technique is best for you. Syringes remain a common method of insulin delivery. They’re the least expensive option, and most insurance companies cover them. Syringes Syringes vary by the amount of insulin they hold and the size of the needle. They’re made of plastic and should be discarded after one use. Traditionally, needles used in insulin therapy were 12.7 millimeters (mm) in length. Recent research shows that smaller 8 mm, 6 mm, and 4 mm needles are just as effective, regardless of body mass. This means insulin injection is less painful than it was in the past. Insulin is injected subcutaneously, which means into the fat layer under the skin. In this type of injection, a short needle is used to inject insulin into the fatty layer between the skin and the muscle. Insulin should be injected into the fatty tissue just below your skin. If you inject the insulin deeper int Continue reading >>
Giving Yourself An Insulin Shot For Diabetes
For those with diabetes, an insulin shot delivers medicine into the subcutaneous tissue -- the tissue between your skin and muscle. Subcutaneous tissue (also called "sub Q" tissue) is found throughout your body. Please follow these steps when using an insulin syringe. Note: these instructions are not for patients using an insulin pen or a non-needle injection system. Select a clean, dry work area, and gather the following insulin supplies: Bottle of insulin Sterile insulin syringe (needle attached) with wrapper removed Two alcohol wipes (or cotton balls and a bottle of rubbing alcohol) One container for used equipment (such as a hard plastic or metal container with a screw-on or tightly secured lid or a commercial "sharps" container) Wash hands with soap and warm water and dry them with a clean towel. Remove the plastic cap from the insulin bottle. Roll the bottle of insulin between your hands two to three times to mix the insulin. Do not shake the bottle, as air bubbles can form and affect the amount of insulin withdrawn. Wipe off the rubber part on the top of the insulin bottle with an alcohol pad or cotton ball dampened with alcohol. Set the insulin bottle nearby on a flat surface. Remove the cap from the needle. If you've been prescribed two types of insulin to be taken at once (mixed dose), skip to the instructions in the next section. Draw the required number of units of air into the syringe by pulling the plunger back. You need to draw the same amount of air into the syringe as insulin you need to inject. Always measure from the top of the plunger. Insert the needle into the rubber stopper of the insulin bottle. Push the plunger down to inject air into the bottle (this allows the insulin to be drawn more easily). Leave the needle in the bottle. Turn the bottle an Continue reading >>
Levemir® Flextouch® Is Ready To Use In Just A Few Steps
Levemir® FlexTouch®, a prefilled insulin pen with no push-button extension, requires low force to inject at all doses and is ready to use in just a few steps.a In fact, Levemir® FlexTouch® has up to 77% less injection force than Lantus® SoloSTAR®. From the makers of the world’s #1-selling prefilled insulin pen,b Levemir® FlexTouch® is: Accurate—Accurate dosing from 1 to 80 units Prefilled—Each pen is prefilled with 300 units of Levemir® Discreet—Fits in your pocket, purse, or nightstand On the go—Take it with you almost anywherec aPlease see the Patient Information for complete Instructions For Use. cOnce in use, Levemir® FlexTouch® must be kept at room temperature below 86°F for up to 42 days. Injecting with Levemir® FlexTouch® You may have concerns about using an injectable medicine for type 2 diabetes. But it’s important to realize the positive effect it may have on the management of your diabetes. And once you gain a little practice in giving injections on your own, Levemir® injections will become part of your daily routine. If you were given instructions from your health care provider on how to use Levemir® FlexTouch® and you have read the Instructions for Use in the Patient Information, you may be ready for your first injection. Your health care provider will tell you what dose of Levemir® is right for you and how many times to take it each day. Your dose may be adjusted based on your blood sugar. Please consult your health care provider prior to adjusting your dose. No compatible source was found for this video. Levemir® can be injected in the thigh, abdomen, or upper arm. It’s important to change the injection site within your injection area each time you inject and not inject into the exact same spot each time. Rotating where yo Continue reading >>
Gestational Diabetes And Injecting Insulin
The insulin is often contained in a pen device with a very small needle that works with a spring. The injections are not usually painful, though they may feel worse if you are anxious. Once people learn to relax, they often find that it is straightforward. Insulin is usually injected into fattier areas such as your tummy, buttocks or thighs. It is important to pick different areas to inject in rotation so that lumps don’t develop under the skin. These can stop the insulin being properly absorbed. Some women feel worried about injecting into their tummy during pregnancy and prefer to use their thighs. In late pregnancy you might find it hard to reach your buttocks so your choice might be limited by where you can reach. Steps to injecting insulin Watch this film from Diabetes UK or follow the steps below Your diabetes team will teach you how to inject insulin, but you can use these steps as a quick reminder. You will need: a pen or syringe, a clean needle, a vial of insulin, a swab to clean the skin, a sharps bin for the used needle. Step 1 Expel two units of insulin into the air to make sure the needle is completely full of insulin. Step 2 Make sure you have the correct dose. Step 3 Decide where you are going to inject. Step 4 If you find the injections painful, rub ice on the area for 20 seconds. Then dry it. Step 5 Gently pinch a fold of skin, if your team has taught you to do this (usually only if you are very slim). Step 6 Put the needle in quickly. Step 7 Inject the insulin, making sure you have pushed down the plunger or button fully. Step 8 Count to ten before pulling the needle out. Step 9 If you pinched a skin fold in Step 4, now let it go. Step 10 Dispose of the needle safely in a sharps bin. Step 11 If you have any problems at all, contact your diabetes team Continue reading >>
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Selected Important Safety Information
Selected Important Safety Information Contraindications Levemir® is contraindicated in patients with hypersensitivity to Levemir® or any of its excipients. Warnings and Precautions Never Share a Levemir® FlexTouch® Between Patients, even if the needle is changed. Sharing poses a risk for transmission of blood-borne pathogens. Dosage adjustment and monitoring: Monitor blood glucose in all patients treated with insulin. Insulin regimens should be modified cautiously and only under medical supervision. Changes in insulin strength, manufacturer, type, or method of administration may result in the need for a change in insulin dose or an adjustment of concomitant anti-diabetic treatment. Administration: Do not dilute or mix with any other insulin or solution. Do not administer subcutaneously via an insulin pump, intramuscularly, or intravenously because severe hypoglycemia can occur. Levemir® (insulin detemir [rDNA origin] injection) Indications and Usage Levemir® (insulin detemir [rDNA origin] injection) is indicated to improve glycemic control in adults and children with diabetes mellitus. Important Limitations of Use Levemir® is not recommended for the treatment of diabetic ketoacidosis. Intravenous rapid-acting or short-acting insulin is the preferred treatment for this condition. Important Safety Information Contraindications Levemir® is contraindicated in patients with hypersensitivity to Levemir® or any of its excipients. Warnings and Precautions Never Share a Levemir® FlexTouch® Between Patients, even if the needle is changed. Sharing poses a risk for transmission of blood-borne pathogens. Dosage adjustment and monitoring: Monitor blood glucose in all patients treated with insulin. Insulin regimens should be modified cautiously and only under medical superv Continue reading >>
22 Best Diabetes And Insulin Videos
Rate this article: (14 votes, average: 4.57 out of 5) Insulin works as a key to the cell, helping to open up its doors to glucose. However where the pancreas cannot produce enough insulin and the body is resistant to it, then Insulin shots may be recommended. Although they may at first have been able to regulate their blood sugar level with regular exercise and a healthy diet, most people with type 2 diabetes will end up needing to take Insulin at some point. Watch this video to learn more. Depending on your work schedule, there are different kinds of insulin to serve your needs. Whilst rapid acting insulin is taken right before or after eating a meal, short acting insulin is taken thirty minutes before eating. Watch this video to find out more about the different types of insulin to fit your work schedule. The benefits of insulin are numerous. Insulin is the oldest natural drug used in the treatment of diabetes. Insulin helps to regulate blood sugar level, preventing it from rising too high or dropping too low. Watch this video to learn more. Insulin on its own rarely causes problems in people with diabetes. However, side effects may occur when insulin is not taken properly. The side effects of insulin include frequent thirst and urge to urinate. On the other hand taking too much insulin can lead to a hypoglycemic reaction. Watch this video to learn more. Do I really need to be on insulin? For many people with type 2 diabetes that have lived most of their life without insulin, a frequent question is, “Is insulin really that important?” If your doctor has put you on insulin then you do. Type 2 diabetes comes with two problems, one your body does not produce enough insulin and two it cannot use the insulin it produces effectively. See this video for more information. Continue reading >>
How To Inject Insulin
Tweet When you are injecting insulin, you should aim to inject into the fatty tissue just underneath the skin. If you think you are injecting into the muscle, you may want to change your technique or ask your GP to prescribe shorter needles. The steps below are a broad guide to injecting insulin. If you plan to change your technique, check with your healthcare team, diabetic nurse or consultant for their advice. How to perform an insulin injection Firstly, prepare your kit. You will need: An insulin pen Enough insulin inside to give the required dose A new pen needle Cotton wool or a tissue Make sure you have your kit available at all times and if possible, inform your family as to its location. If you suffer from a hypo, this will allow your family to act quickly. Injecting your insulin shot To perform your insulin injection: Wherever possible, wash your hands with soap and water before injecting Put a new needle onto your pen Perform an ‘air shot’ of at least 2 units to clear any bubbles out of the needle – if you do not get a steady stream, repeat the air shot until you do get a steady stream Dial up your dose – how you do this exactly may depend on which pen you have Pick a soft fatty area to inject – tops of thighs, belly, bum and triceps (not always recommended for children or thinner people) Raise a fold of fatty flesh slightly between your thumb and fingers - leaving plenty of space between to put the needle in Put the needle in – if you are particularly slim, you may need to put the needle in at a 45 degree angle to avoid injecting into the muscle Push the plunger, to inject the dose, relatively slowly After the dose has been injected, hold the needle in for a good 10 seconds to prevent too much insulin from escaping out If any blood or insulin esca Continue reading >>
How To Give Your Diabetic Cat An Insulin Injection
Learn how to care for your pet with help from VetVid. See how to give your diabetic cat an insulin injection with this video tutorial. In this video, Dr. Mike educates you on the importance of using the proper syringe for your concentration of insulin. He also shows you an easy way to give your diabetic cat an insulin injection. VetVid's videos assist pet owners in making informed decisions on healthcare issues for their animals. Their veterinary advice is for consumers around the world! But... this information is not meant to replace the advice of your regular veterinarian. VetVid = Good advice for healthier pets! Check out more VetVid videos on WonderHowTo. Continue reading >>
How To Use An Insulin Pen
Whether you're at home or on the go, an insulin pen offers the benefits of accuracy, convenience, and confidence to people with diabetes. Here's how to use one safely and easily. Whether you're at home or on the go, an insulin pen offers the benefits of accuracy, convenience, and confidence to people with diabetes. Here's how to use one safely and easily. Whether you're at home or on the go, an insulin pen offers the benefits of accuracy, convenience, and confidence to people with diabetes. Here's how to use one safely and easily. Whether you're at home or on the go, an insulin pen offers the benefits of accuracy, convenience, and confidence to people with diabetes. Here's how to use one safely and easily. Continue reading >>
Video: How To Inject Insulin For Diabetes
Are you nervous about injecting insulin for the first time? With a little practice you'll discover it only takes a few seconds. Watch our how-to video to help ease your anxiety. A transcript of this video is also available. More videos on living with diabetes: Read the transcript of the video, "How to Inject Insulin for Diabetes" below: Hi. My name is Jenny De Jesus, and I’m a registered nurse and certified diabetes educator. If you, or a loved one, has diabetes and has been prescribed insulin, there are several things you should be aware of: To begin, many types of insulin are available. Your health care provider will decide the best type, amount and administration times for you. It’s also important to know the ways in which insulin may be injected and which parts of the body are potential injection sites. It’s understandable if you’re nervous or anxious about injecting insulin for the first time. Although injecting insulin might feel awkward or scary at first, with a little practice you'll discover it only takes a few seconds. To help ease your anxiety, I’m going to show you how. Let’s get started. There are three primary tools used to inject insulin. They are: Insulin syringes Insulin pens And insulin pumps. There are four areas of the body in which insulin is injected. They are: The abdominal area Some parts of your arms Some parts of your legs And certain areas of the buttocks. The preferred injection site is the abdomen, specifically from the bottom of the ribs to the pubic line, avoiding the navel. The abdomen provides the best absorption and is relatively easy to reach. Avoid areas with scarring or moles because the tough tissue may not absorb insulin well. If your health care provider has prescribed insulin for you to inject using a syringe and vial Continue reading >>
How To Give A Shot Of Insulin For Dogs In 3 Steps
When you have a diabetic dog, changing their diet and lifestyle can seem like the "easy" part compared to having to give your dog a daily insulin shot. Here are 3 easy steps to administering an insulin shot. Take heart, it's actually easier than it looks. If your dog has been diagnosed with canine diabetes, your vet has probably prescribed insulin injections. Insulin is a hormone that helps regulate the body’s blood sugar, or glucose, levels. Dogs with diabetes can only regulate their blood sugar with insulin injections, so you’ll have to learn to give a shot of insulin to your dog. Diabetes affects as many as 1 in 500 dogs. It’s a common health problem, and one that is manageable with consistent treatment and lifestyle changes. Many pet parents are understandably nervous about giving their dog shots, but when properly given they cause only minimal discomfort. Once you master these steps, the process will be a quick part of your—and your dog’s—routine. Your veterinarian will give you the proper dosage and the number of shots a day your dog needs –it’s important to give the injections at the same time each day. Step 1. Store the insulin carefully Insulin can be a fragile substance. It should not be exposed to direct sunlight or stored in high temperatures. Keep your unused bottles in the refrigerator, not frozen. Storing it in the fridge door is often recommended. If the insulin bottle looks frosted, was possibly exposed to heat, or the liquid seems unevenly colored, start with a new vial to be safe. Do not use insulin past the expiration date on the bottle. TIP: Although insulin is sensitive to extreme temperatures, bringing it to room temperature before use will not harm the hormone and may be more comfortable at the injection site for your pet. Step 2. Continue reading >>
How To Inject Insulin
Giving yourself an insulin jab may sound daunting, but with practice, you will soon learn to handle it with ease To prepare for an insulin injection, you need to: fill the syringe with the right amount of insulin decide which part of your body to inject know how to inject Step 1: Get Ready Have everything you need before you inject. Get ready your insulin, needles, syringes, alcohol wipes and a container for throwing away used needles and syringes. Step 2: Decide Where to Inject Insulin works best when you inject it into the fatty parts of your body just under the skin. If you inject insulin too deep into the muscle, insulin will enter the blood too quickly. This can cause your blood glucose levels to drop too low. Each time you inject insulin, you should select a different spot on your body. For example, you can inject different areas of your abdomen. You can also inject your upper arm, thigh or buttocks. To help you remember, keep a chart of the areas you have injected. Keep injection sites about an inch apart, and away from scars and your belly button. Do not inject into areas that are bruised, swollen or painful. Step 3: How to Inject Once you’ve selected the injection site, follow these steps: Clean and dry the injection site. Pinch up a large area of the skin around the injection site. With your other hand, insert the needle at a 90 degree angle. However, a 45 degree angle may be used in children or in an area where there is little fat. Push the needle all the way into the skin. Release the pinch on your skin and slowly push the plunger all the way in to inject the insulin. Pull out the needle. Return the used needle and syringe to the container and throw it away safely. Never reuse needles or syringes. How to Inject Insulin (Video) Different Ways to Inject I Continue reading >>
8 Ways To Take Insulin
How to take insulin Need insulin? While the drug itself may be old—nearly 90 years to be exact—there’s lots of new things happening when it comes to ways to take it. From the old-fashioned needle and syringe to injector pens to pumps, you’ve got choices to make. There’s even a plethora of devices that can help you inject if you have poor vision or mobility issues. Check out these eight options and talk with your certified diabetes educator to determine which insulin delivery system or injection aids are right for you. Needle and syringe With this type of delivery system, you insert a needle into a vial, draw up the appropriate amount of insulin, and then inject into the subcutaneous space—the tissue just under your skin. Here are 5 types of insulin and 9 factors that affect how insulin works. Even though there are other options, needles and syringes remain the most common way to take insulin. Some of the new insulin injection methods, such as the insulin pen, carry only a preset amount of insulin. Thinner needles and other advancements, such as syringe magnifiers, have made syringes easier to use. Syringe magnifier Have poor vision? You’re not alone. According to the American Diabetes Association, diabetes is the leading cause of new cases of blindness among adults aged 20–74 years. Needle guides can help you keep the syringe or pen steady at the desired location and at the correct angle both for drawing up insulin out of the vial and injecting. Some needle guides also come with magnifiers, which help by enlarging the numbers and allowing you to read the fine print and dosages on the syringe. Syringe-filling device These devices are another example of innovations designed to help make insulin needles more palatable. Syringe-filling devices allow a person Continue reading >>
So You’re Ready
Indication BASAGLAR is a long-acting insulin used to control high blood sugar in adults and children with type 1 diabetes and adults with type 2 diabetes. Limitation of Use Important Safety Information Do not take BASAGLAR during episodes of low blood sugar or if you are allergic to insulin glargine or any of the ingredients in BASAGLAR. Do NOT reuse needles or share insulin pens, even if the needle has been changed. Before starting BASAGLAR, tell your doctor about all your medical conditions, including if you have liver or kidney problems, if you are pregnant or planning to become pregnant, or if you are breastfeeding or planning to breastfeed. BASAGLAR should be taken once a day at the same time every day. Test your blood sugar levels while using insulin. Do not make any changes to your dose or type of insulin without talking to your healthcare provider. Any change of insulin should be made cautiously and only under medical supervision. The most common side effect of insulin, including BASAGLAR, is low blood sugar (hypoglycemia), which may be serious and life threatening. Signs and symptoms may include dizziness or light-headedness, sweating, confusion, headache, blurred vision, slurred speech, shakiness, fast heartbeat, anxiety, irritability, mood change, or hunger. Do NOT dilute or mix BASAGLAR with any other insulin or solution. It will not work as intended and you may lose blood sugar control, which could be serious. BASAGLAR must only be used if the solution is clear and colorless with no particles visible. Always make sure you have the correct insulin before each injection. BASAGLAR may cause serious side effects that can lead to death, such as severe allergic reactions. Get emergency help if you have: Heart failure can occur if you are taking insulin together w Continue reading >>
