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Streptozotocin Nicotinamide Induced Rat Model Of Type 2 Diabetes

Eugenosedin-a Improves Glucose Metabolism And Inhibits Mapks Expression In Streptozotocin/nicotinamide-induced Diabetic Rats - Sciencedirect

Eugenosedin-a Improves Glucose Metabolism And Inhibits Mapks Expression In Streptozotocin/nicotinamide-induced Diabetic Rats - Sciencedirect

Eugenosedin-A improves glucose metabolism and inhibits MAPKs expression in streptozotocin/nicotinamide-induced diabetic rats Author links open overlay panel Kuo-PingShena Hui-LiLinb Hsueh-WeiYenc Su-LingHsiehd Li-MeiAne Bin-NanWuefg Open Access funded by Kaohsiung Medical University This study examined the effects of eugenosedin-A (Eu-A) in a streptozotocin (STZ)/nicotinamide-induced rat model of type II diabetes mellitus (T2DM). Six-week-old SpragueDawley rats were randomly divided into three groups: (1) RD group, normal rats fed a regular diet (RD), (2) DM group, T2DM rats fed a high-fat diet, and (3) Eu-A group, T2DM rats fed a high fat diet plus oral Eu-A (5mg/kg/day). After 30 days, the DM group had higher body weight, higher blood glucose and lower insulin levels than the RD group. The DM group also had increased protein expression of glycogen synthase kinase (GSK) in liver and skeletal muscle and decreased protein expression of insulin receptor (IR), insulin receptor substrate-1 (IRS-1), IRS-2, AMP-activated protein kinase (AMPK), glucose transporter-4 (GLUT-4), glucokinase (GCK), and peroxisome proliferator-activated receptor (PPAR-). STZ/nicotinamide-induced T2DM increased the expression of mitogen-activated protein kinases (MAPKs: p38, ERK, JNK) and inflammatory p65 protein. In the Eu-A treated T2DM rats, however, blood glucose was attenuated and the insulin concentration stimulated. Changes in IR, IRS-1 and IRS-2 proteins as well as AMPK, GLUT-4, GCK, GSK, PPAR-, MAPKs, and inflammatory p65 proteins were ameliorated. These results suggested that Eu-A alleviates STZ/nicotinamide-induced hyperglycemia by improving insulin levels and glucose metabolism, and inhibiting the MAPKs- and p65-mediated inflammatory pathway. Continue reading >>

Modulatory Effects Of Garlic, Ginger, Turmeric And Their Mixture On Hyperglycaemia, Dyslipidaemia And Oxidative Stress In Streptozotocinnicotinamide Diabetic Rats

Modulatory Effects Of Garlic, Ginger, Turmeric And Their Mixture On Hyperglycaemia, Dyslipidaemia And Oxidative Stress In Streptozotocinnicotinamide Diabetic Rats

This article has been cited by the following publications. This list is generated based on data provided by CrossRef . Zhu, JieChen, HaoSong, ZhixiuWang, XudongandSun, Zhenshuang2018.Effects of Ginger (Zingiber officinale Roscoe) on Type 2 Diabetes Mellitus and Components of the Metabolic Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.Evidence-Based Complementary and Alternative Medicine,Vol. 2018,Issue. ,p.1. Taheri Rouhi, Seyedeh ZeinabSarker, Md. Moklesur RahmanRahmat, AsmahAlkahtani, Saad AhmedandOthman, Fauziah2017.The effect of pomegranate fresh juice versus pomegranate seed powder on metabolic indices, lipid profile, inflammatory biomarkers, and the histopathology of pancreatic islets of Langerhans in streptozotocin-nicotinamide induced type 2 diabetic SpragueDawley rats.BMC Complementary and Alternative Medicine,Vol. 17,Issue. 1, View all Google Scholar citations for this article. Modulatory effects of garlic, ginger, turmeric and their mixture on hyperglycaemia, dyslipidaemia and oxidative stress in streptozotocinnicotinamide diabetic rats College of Clinical Pharmacy, King Faisal University College of Science, King Faisal University Spices which show hypoglycaemic, hypolipidaemic and antioxidant activities may have a role in the treatment of diabetes and its complications. The present study aimed to compare the modulatory effects of garlic, ginger, turmeric and their mixture on the metabolic syndrome and oxidative stress in streptozotocin (STZ)nicotinamide diabetic rats. Diabetes was induced in overnight fasted rats by a single intraperitoneal injection of STZ (65mg/kg body weight) and nicotinamide (110mg/kg body weight, 15min before STZ injection). Diabetic rats orally received either distilled water (as vehicle) or 200mg/kg Continue reading >>

Streptozotocininduced Diabetic Models In Mice And Rats

Streptozotocininduced Diabetic Models In Mice And Rats

Please review our Terms and Conditions of Use and check box below to share full-text version of article. I have read and accept the Wiley Online Library Terms and Conditions of Use. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Streptozotocin (STZ) is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZinduced insulin deficiency and hyperglycemia in mice and rats. Also described are protocols for creating animal models for type 2 diabetes using STZ. These animals are employed for assessing the pathological consequences of diabetes and for screening potential therapies for the treatment of this condition. 2015 by John Wiley & Sons, Inc. Yu-Ting Tseng, Wan-Hsuan Chang, Chih-Cheng Lin, Fang-Rong Chang, Pao-Chu Wu and Yi-Ching Lo, Protective effects of Liuwei dihuang water extracts on diabetic muscle atrophy, Phytomedicine, 10.1016/j.phymed.2018.09.032, 53, (96-106), (2019). Liming Yu, Zhi Li, Xue Dong, Xiaodong Xue, Yu Liu, Shu Xu, Jian Zhang, Jinsong Han, Yang Yang and Huishan Wang, Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling, Oxidative Medicine and Cellular Longevity, 2018, (1), (2018). Haiyan Wei, Qiaoyun Hu, Junxia Wu, Chenjuan Yao, Lingfei Xu, Fengjun Xing, Xinyuan Zhao, Shali Yu, Xiaoke Wang and Gang Chen, Molecular mechanism of the increased tissue uptake of trivalent inorganic arsenic in mice with type 1 diabetes mellitus, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2018.06.029, 504, 2, (393-399), (2018). Jay Parikh, Alice Zemljic-Harpf, Johnny F Continue reading >>

Prime Pubmed | Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes (review

Prime Pubmed | Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes (review

Type your tag names separated by a space and hit enter Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Diabetes is one of the five leading causes of death in the world, with type 2 diabetes occurring more frequently than type 1. Management of diabetes without side effects is still a challenge and therefore new strategies need to be examined. Because of difficulties in human research, animal models of diabetes are useful research tools for this purpose and rodent models of type 2 diabetes are the first choice. The aim of this study is an overview on one of the most frequently used models of type 2 diabetes in rat, induced by streptozotocin and nicotinamide, considering its advantages and disadvantages for diabetes research in humans. Ghasemi A, Khalifi S, Jedi S: "Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review)." Acta physiologica Hungarica, vol. 101, no. 4, 2014, pp. 408-20, Accessed November 29, 2018. Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta Physiol Hung 2014;101(4):408-20 Accessed November 29, 2018. Ghasemi A & Khalifi S & Jedi S. (2014). Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta physiologica Hungarica, 101, pp. 408-20. doi:10.1556/APhysiol.101.2014.4.2 Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta Physiol Hung. 2014;101:408-20 TY - JOURT1 - Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review).AU - Ghasemi,Asghar,AU - Khalifi,S,AU - Jedi,S,PY - 2014/12/24/entrezPY - 2014/12/24/pubmedPY - 2015/3/12/medlineKW - animal modelKW - nicotinamideKW - ratKW - streptozotocinKW - type 2 diabetesSP - 408EP - 20JF - Acta physiologica Hunga Continue reading >>

Magnesium Upregulates Insulin Receptor And Glucose Transporter-4 In Streptozotocin-nicotinamide-induced Type-2 Diabetic Rats

Magnesium Upregulates Insulin Receptor And Glucose Transporter-4 In Streptozotocin-nicotinamide-induced Type-2 Diabetic Rats

Magnesium upregulates insulin receptor and glucose transporter-4 in streptozotocin-nicotinamide-induced type-2 diabetic rats Magnesium upregulates insulin receptor and glucose transporter-4 in streptozotocin-nicotinamide-induced type-2 diabetic rats Department of Biochemistry, College of Medicine, University of Lagos, Nigeria Department of Physiology, Benjamen S. Carson (Snr) School of Medicine, Babcock University, Babcock, Nigeria Published Online: 2018-02-16 | DOI: Objective. We investigated the effects of magnesium supplementation on glucose tolerance, insulin sensitivity, oxidative stress as well as the concentration of insulin receptor and glucose transporter-4 in streptozotocin-nicotinamide induced type-2 diabetic (T2D) rats. Methods. Rats were divided into four groups designated as: 1) control (CTR); 2) diabetic untreated (DU); 3) diabetic treated with 1 mg of Mg/kg diet (Mg1-D); and 4) diabetic treated with 2 mg of Mg/kg diet (Mg2-D). T2D was induced with a single intraperitoneal (i.p.) injection of freshly prepared streptozotocin (55 mg/kg) aft er an initial i.p. injection of nicotinamide (120 mg/kg). Glucose tolerance, insulin sensitivity, lipid profile, malondialdehyde (MAD) and glutathione content, insulin receptors (INSR) and glucose transporter-4 (GLUT4), fasting insulin and glucose levels were measured, and insulin resistance index was calculated using the homeostatic model assessment of insulin resistance (HOMA-IR). Results. Magnesium supplementation improved glucose tolerance and lowered blood glucose levels almost to the normal range. We also recorded a noticeable increase in insulin sensitivity in Mg-D groups when compared with DU rats. Lipid perturbations associated T2D were significantly attenuated by magnesium supplementation. Fasting glucose leve Continue reading >>

The Hypoglycemic Activity Of Euclea Undulata Thunb. Var. Myrtina (ebenaceae) Root Bark Evaluated In A Streptozotocin-nicotinamide Induced Type 2 Diabetes Rat Model

The Hypoglycemic Activity Of Euclea Undulata Thunb. Var. Myrtina (ebenaceae) Root Bark Evaluated In A Streptozotocin-nicotinamide Induced Type 2 Diabetes Rat Model

The hypoglycemic activity of Euclea undulata Thunb. var. myrtina (Ebenaceae) root bark evaluated in a streptozotocin-nicotinamide induced type 2 diabetes rat model JavaScript is disabled for your browser. Some features of this site may not work without it. The hypoglycemic activity of Euclea undulata Thunb. var. myrtina (Ebenaceae) root bark evaluated in a streptozotocin-nicotinamide induced type 2 diabetes rat model Deutschlander, M.S. (Miranda Susan); Lall, Namrita; Van de Venter, Maryna; Dewanjee, S. The hypoglycaemic activity of a crude acetone extract of the root bark of Euclea undulata var. myrtina was evaluated in a streptozotocin-nicotinamide induced type 2 diabetes rat model after positive results were obtained by in vitro screening of glucose utilization by C2C12 myocytes, 3T3-L1 preadipocytes and Chang liver cells and alpha-glucosidase inhibition. Thirty male Wistar rats were used for the experiment. Type 2 diabetes was induced by a single intraperitoneal injection of streptozotocin and administration of nicotinamide 15 minutes after. Animals exhibiting fasting glucose levels of 140-200 mg/dl after 7 days were screened as type 2 diabetes. Extract was administered for 21 days orally at a concentration of 50 mg/kg and 100 mg/kg respectively. Glibenclamide (1mg/kg) was used as positive control. On day 21, blood lipid profiles and body weight were determined by using standard enzymatic colorimetric kits before the rats were sacrificed by cervical decapitation. The crude acetone extract of E. undulata root bark at a concentration of 100mg/kg body weight significantly lowered fasting blood glucose levels as well as elevated cholesterol and triglyceride levels to near normal without any weight gain. The results indicate that the crude acetone root bark extract of E Continue reading >>

Streptozotocin-nicotinamide-induced Diabetic Model

Streptozotocin-nicotinamide-induced Diabetic Model

Home / Lead Optimization / Pharmacology and Pharmacodynamics Studies / Rodent Models / Non-Genetically Engineering Models (NON-GEMs) / Rodent Metabolic Disease Models / Streptozotocin-Nicotinamide-Induced Diabetic Model Streptozotocin-Nicotinamide-Induced Diabetic Model As one of the most reliable industry leaders in developing animal models of diseases, Creative Biolabs provides a large range of chemically-induced experimental diabetic mice/rats models coupled with comprehensive relevant services. Our seasoned scientists are pleased to offer custom service to meet your specific requirements. Numerous efforts are constantly being made not only to investigate diabetes and diabetes-related complications but also to test new therapeutics for diabetes. Accordingly, a large number of animal models exist, making it necessary for investigators to carefully consider and compare before they choose the most suitable model because of their differential utility in reflecting various aspects of diabetic abnormalities. In one of these frequently used models, diabetes is induced by injection of both streptozotocin (STZ) and nicotinamide (NA) to adult rats or mice, which was first established in 1998. In the process of induction, two chemical compounds are given: STZ and NA. STZ, a nitrosourea analogue, is a well-known diabetogenic agent that will damage pancreatic -cells due to DNA alkylation. However, NA, the amide form of vitamin B3, partially protects these cells against the detrimental effects of STZ via attenuation of DNA damage, proinsulin biosynthesis, body weight loss, diminished pancreatic insulin content and so on. Main Characteristics of STZ-NA-Induced Diabetes Model Firstly, doses of STZ and NA must be adjusted to obtain the appropriate severity in this diabetic model. In Continue reading >>

Streptozotocin-nicotinamide-induced Diabetes In The Rat. Characteristics Of The Experimental Model.

Streptozotocin-nicotinamide-induced Diabetes In The Rat. Characteristics Of The Experimental Model.

Exp Biol Med (Maywood). 2012 May;237(5):481-90. doi: 10.1258/ebm.2012.011372. Epub 2012 May 22. Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland. [email protected] Administration of both streptozotocin (STZ) and nicotinamide (NA) has been proposed to induce experimental diabetes in the rat. STZ is well known to cause pancreatic B-cell damage, whereas NA is administered to rats to partially protect insulin-secreting cells against STZ. STZ is transported into B-cells via the glucose transporter GLUT2 and causes DNA damage leading to increased activity of poly(ADP-ribose) polymerase (PARP-1) to repair DNA. However, exaggerated activity of this enzyme results in depletion of intracellular NAD(+) and ATP, and the insulin-secreting cells undergo necrosis. The protective action of NA is due to the inhibition of PARP-1 activity. NA inhibits this enzyme, preventing depletion of NAD(+) and ATP in cells exposed to STZ. Moreover, NA serves as a precursor of NAD(+) and thereby additionally increases intracellular NAD(+) levels. The severity of diabetes in experimental rats strongly depends on the doses of STZ and NA given to these animals. Therefore, in diabetic rats, blood glucose may be changed in a broad range--from slight hyperglycemia to substantial hyperglycemia compared with control animals. Similarly, blood insulin may be only slightly decreased or substantial hypoinsulinemia may be induced. In vitro studies demonstrated that the insulin-secretory response to glucose is attenuated in STZ-NA-induced diabetic rats compared with control animals. This is due to reduced B-cell mass as well as metabolic Continue reading >>

Epigallocatechin-3-gallate Protects Against Diabetic Cardiomyopathy Through Modulating The Cardiometabolic Risk Factors, Oxidative Stress, Inflammation, Cell Death And Fibrosis In Streptozotocin-nicotinamide-induced Diabetic Rats.

Epigallocatechin-3-gallate Protects Against Diabetic Cardiomyopathy Through Modulating The Cardiometabolic Risk Factors, Oxidative Stress, Inflammation, Cell Death And Fibrosis In Streptozotocin-nicotinamide-induced Diabetic Rats.

The potential protective effect of epigallocatechin-3-gallate (EGCG) on type 2 diabetes-induced heart injury was investigated. A rat model of diabetes was achieved by injection of nicotinamide (100mg/kg, i.p), 20min before the administration of streptozotocin (55mg/kg, i.p.). After confirmation of diabetes, EGCG (2mg/kg, p.o.) was administrated on alternate days for one month. Treatment of diabetic rats with EGCG showed a remarkable reduction in glucose, glycosylated hemoglobin, HOMA-IR and lipid profile levels with an elevation in insulin levels, indicating its antihyperglycemic and antidyslipidemic actions. EGCG treatment also suppressed the increase in the levels of superoxide, 4-hydroxynonenal and protein carbonyl, whereas it increased the content of glutathione and the activities of superoxide dismutase and catalase in heart of diabetic rats, indicating its antioxidant capacity. In addition, EGCG improved heart function of diabetic rats as evidenced by a remarkable reduction in troponin T level and creatine kinase-MB, lactate dehydrogenase and aspartate aminotransferase activities in the serum. Oral administration of EGCG for one month after diabetes induction significantly protected the increase in serum levels of pro-inflammatory cytokines (IL-1 , IL-6 and TNF-) and adhesion molecules (ICAM-1 and VCAM-1), suggesting its anti-inflammatory potential. Furthermore, EGCG hampered the mitochondrial apoptotic pathway through increasing Bcl-2 level and decreasing p53, Bax, cytochrome c and caspase-3 and 9 levels in hearts of diabetic rats, indicating its anti-apoptotic action. Diabetic rats treated with EGCG also exhibited decreased level of DNA damage in the myocardium. The histological examinations indicated the cardioprotective effect of EGCG against harmful impact o Continue reading >>

Hypoglycemic And Antioxidative Effects Of Glossogyne Tenuifolia On Streptozotocin-nicotinamide-induced Diabetic Rats

Hypoglycemic And Antioxidative Effects Of Glossogyne Tenuifolia On Streptozotocin-nicotinamide-induced Diabetic Rats

Hypoglycemic and Antioxidative Effects of Glossogyne tenuifolia on Streptozotocin-Nicotinamide-Induced Diabetic Rats Download as PDF (Size:632KB) PP. 1170-1181 DOI: 10.4236/ajps.2017.85077 588 Downloads 959 Views Citations Glossogyne tenuifolia (GT) is the traditional herbal tea in Penghu Island, Taiwan. Recent research hasshown that the active components in GT are potential inhibitors of -glucosidase.The present study investigated that whether or not GT could improve the statusof type 2 diabetes mellitus. Male Wistar rats aged eight weeks were induced tobe hyperglycemic by subcutaneous injection of streptozotocin-nicotinamide (STZ-NA) andcombination of high-fat diet (HFD). The animals were given GT extractsat the low dose or high dose, or the anti-diabetic drug (acarbose), in drinkingwater for 4 weeks. The results showed that hot water extracts from GT resultedin significantly decreases in fasting blood glucose at the 1st and 2nd weeks,fasting insulin levels at the 2nd week, 1 hour postprandial blood glucose after the starch tolerance test on Day 23 and blood glucose levels after oral glucose tolerancetest (OGTT) at the 60th minute on Day 25. In addition, diabetic rats treated with GT extractsfrom hot water for 4 weeks displayed significantly decreased thiobarbituricacid reactive substances (TBARS) in the serum, liver and kidney, serum totalcholesterol, fasting insulin levels and homeostasis model assessment forinsulin resistance (HOMA-IR). Overall, these resultsdemonstrate that the hot waterextracts of GT might improve the progression of diabetes and decrease oxidativestress in STZ-NA-induceddiabetic rats. Glossogyne tenuifolia , Blood Glucose , Antioxidative , Diabetic Rats Diabetes mellitus (DM) is currently recognized as a chronic metabolic disease with the highes Continue reading >>

Effect Of Atorvastatin On Pharmacology Of Sitagliptin In Streptozotocin-nicotinamide Induced Type-ii Diabetes In Rats

Effect Of Atorvastatin On Pharmacology Of Sitagliptin In Streptozotocin-nicotinamide Induced Type-ii Diabetes In Rats

Received date: December 17, 2014; Accepted date: December 31, 2014; Published date: January 07, 2015 Citation: Eggadi V, Sheshagiri SBB, Devandla A, Dasi N, Kulundaivelu U, et al. (2015) Effect of Atorvastatin on Pharmacology of Sitagliptin in Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats. Biol Med 7:225. doi:10.4172/0974-8369.1000225 Copyright: 2015 Eggadi V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Prolonged type 2 diabetes mellitus (Type2DM) may lead to high risk of cardiovascular disease (CVD) requiring a global therapeutic approach. Statin therapy has proven its efficacy in reducing CVD events in Type2 DM patients. Dipeptidyl peptidase-4 inhibitors (gliptins), which are increasingly used to target hyperglycemia. In the present study type 2 DM in rats by i.p. Administration of Streptozotocin (STZ); (60 mg/kg) -Nicotinamide (120 mg/kg). Diabetes induced rats were divided into groups and treated with Sitagliptin alone and in combination with atorvastatin for 7 days. Blood samples were collected by retro orbital puncture. Mean glucose concentration was measured by GODPOD method using commercial glucose kits and sitagliptin in plasma was estimated by RP-HPLC method using methanol: water (60:40 v/v, containing 10 mM Tris and 10 mM Triethylamine) was adjusted to pH 9.0 using 1 mol/L hydrochloric acid. The blood glucose lowering activity of sitagliptin was increased by the presence of atorvastatin in diabetic rats. The pharmacokinetic (PK) and pharmacodynamic (PD) parameters of sitagliptin in diabetic rats were significantly changed in the presence of atorvas Continue reading >>

Effect Of Eugenia Jambolana On Streptozotocin-nicotinamideinduced Type-2 Diabetic Nephropathy In Rats

Effect Of Eugenia Jambolana On Streptozotocin-nicotinamideinduced Type-2 Diabetic Nephropathy In Rats

Effect of Eugenia Jambolana on Streptozotocin-Nicotinamideinduced type-2 Diabetic Nephropathy in Rats Godwin Selvaraj Esther1*, Alvin Jose Manonmani2 Research Scholar, PRIST University, Vallam, Thanjavur- 613403, Tamil Nadu, India./ Department of Pharmacology, Noorul Islam College of Dental Science, NIMS Medicity, Aralummoodu-695123, Trivandrum, Kerala, India. Department of Pharmacology, Swamy Vivekanandha College of Pharmacy, Elayampalayam, Tiruchengode-637205, Tamil Nadu, India. Corresponding Author: Godwin Selvaraj Esther Department of Pharmacology, Noorul Islam College of Dental Science, NIMS Medicity, Aralummoodu-695123, Trivandrum, Kerala, India. E-mail: [email protected] Date of Submission: 08-12-2013 Date of Acceptance: 17-01-2014 Conflict of Interest: NIL Source of Support: NONE Copyright: 2014 Godwin Selvaraj Esther et al, publisher and licensee IYPF. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. Related article at Pubmed , Scholar Google Visit for more related articles at International Journal of Drug Development and Research The chronic type-2 diabetes mellitus leads to diabetic nephropathy, which is one of the major microvascular complication of end stage renal disease worldwide and causes premature death in diabetic patients. The objective of the present investigation was to evaluate the antidiabetic activity and protective effect of diabetic induced nephropathy of ethanolic extract of seeds of Eugenia jambolana (SEEJ) by using in-vitro and in-vivo models. The in-vitro antidiabetic effect was studied by glucose uptake assay in lymphocyte culture preparation. The in-vivo antidiabetic activity and the effect on diabetic nephropathy was evaluated using streptozotocin-nicotinam Continue reading >>

Ghasemi, A., Khalifi, S., Jedi, S. Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes. Acta Physiol Hung. 101, 408-420, 2014.

Ghasemi, A., Khalifi, S., Jedi, S. Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes. Acta Physiol Hung. 101, 408-420, 2014.

Ghasemi, A., Khalifi, S., Jedi, S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes. Acta Physiol Hung. 101, 408-420, 2014. 1Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3 Kampus UGM, Bulaksumur, Yogyakarta 55281, Indonesia 2Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia 3Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia Copyright 2015 Science and Education Publishing Nurliyani, Eni Harmayani, Sunarti. Nitric Oxide and Inflammatory Cytokine Productions in Diabetic Rats Supplemented with Goat Milk and Soy Milk Kefir. Journal of Food and Nutrition Research. 2015; 3(6):384-391. doi: 10.12691/jfnr-3-6-5. Correspondence to: Nurliyani, Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3 Kampus UGM, Bulaksumur, Yogyakarta 55281, Indonesia. Email: [email protected] Diabetes has been known as an inflammatory diseases, and the certain of probiotics when administered in adequate amounts were reported have an anti-inflammatory properties. The purpose of this study were to evaluate the effect of administration of goat milk and soy milk kefir on immune responses including spleen lymphocyte proliferation, production of pro-inflammatory cytokine (TNF-) and anti-inflammatory cytokine (IL-10), as well as peritoneal macrophage nitric oxide (NO) production in diabetic rats induced with streptozotocin-nicotinamide. Male Wistar rats were divided into five groups: negative control, positive control, diabetic rats fed goat milk kefir, diabetic rats fed kefir combination from goat milk and soy milk kefir and diabetic rats fed soy milk kefir. After 5 weeks treatment, the rats were sacrif Continue reading >>

Non-genetic Rat Model Of Nephropathy In Type 2 Diabetes With Attenuated Streptozotocin-induced Tubular Alteration

Non-genetic Rat Model Of Nephropathy In Type 2 Diabetes With Attenuated Streptozotocin-induced Tubular Alteration

Endocrine Abstracts (2017) 49 GP92 | DOI: 10.1530/endoabs.49.GP92 Non-genetic rat model of nephropathy in type 2 diabetes with attenuated streptozotocin-induced tubular alteration Valentina Bayrasheva1,2, Alina Babenko1,2, Ivan Pchelin3, Anna Arefjeva2, Svetlana Chefu1,2, Ivan Shatalov4, Yurii Dmitriev1, Alexandra Ivanova3,5, Pavel Andoskin1, Parvis Aliev2 & Elena Grineva1,2 1Federal Almazov North-West Medical Research Centre, Saint Petersburg, Russia; 2Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia; 3Saint Petersburg State University, Saint Petersburg, Russia; 4Saint Petersburg National Research University of Information, Technologies, Mechanics and Optics, Saint Petersburg, Russia; 5Komarov Botanical Institute of the Russian Academy of Sciences, Saint Petersburg, Russia. Non-genetic animal models of diabetic nephropathy (DN) are most commonly reproduced by using streptozotocin (STZ) which preferentially gets into -cells via GLUT2 transporters. However, STZ administration results in nephrotoxic effects as well, due to expression of GLUT2 by renal tubular epithelial cells. We hypothesized that nicotinamide (NA), which is considered to attenuate the severity of STZ-induced -cell damage, could also prevent tubular alteration. Starting at 3 weeks after unilateral nephrectomy, thirty adult male Wistar rats were fed the high-fat diet for 5 weeks and then successively received either NA (230 mg/kg) and STZ (65 mg/kg, NA-STZ-group) or STZ in a low dose (40 mg/kg, LD-STZ-group) intraperitoneally in 15-min interval. Control nondiabetic uninephrectomized rats received vehicle and were fed normal chow (C-group). At weeks 10, 20, and 30 (the end of the study), metabolic parameters, creatinine clearance, albuminuria, and urinary tubular injury mark Continue reading >>

Streptozotocin-nicotinamide Induced Diabetic: Topics By Science.gov

Streptozotocin-nicotinamide Induced Diabetic: Topics By Science.gov

Barik, Rakesh; Jain, Sanjay; Qwatra, Deep; Joshi, Amit; Tripathi, Girraj Sharan; Goyal, Ravi Objective: To evaluate the antidiabetic activity of aqueous extract of roots of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats. Materials and Methods: Streptozotocin-nicotinamide induced type-II diabetic rats (n = 6) were administered aqueous root extract (250 and 500 mg/kg, p.o.) of Ichnocarpus frutescens or vehicle (gum acacia solution) or standard drug glibenclamide (0.25 mg/kg) for 15 days. Blood samples were collected by retro-orbital puncture and were analyzed for serum glucose on days 0, 5, 10, and 15 by using glucose oxidase-peroxidase reactive strips and a glucometer. For oral glucose tolerance test, glucose (2 g/kg, p.o.) was administered to nondiabetic control rats and the rats treated with glibenclamide (10 mg/kg, p.o.) and aqueous root extract of Ichnocarpus frutescens. The serum glucose levels were analyzed at 0, 30, 60, and 120 min after drug administration. The effect of the extract on the body weight of the diabetic rats was also observed. Results: The aqueous root extract of Ichnocarpus frutescens (250 and 500 mg/kg, p.o.) induced significant reduction (P < 0.05) of fasting blood glucose levels in streptozotocin-nicotinamide induced type-II diabetic rats on the 10th and 15th days. In the oral glucose tolerance test, the extract increased the glucose tolerance. It also brought about an increase in the body weight of diabetic rats. Conclusion: It is concluded that Ichnocarpus frutescens has significant antidiabetic activity as it lowers the fasting blood sugar level in diabetic rats and increases the glucose tolerance. PMID:21264156 Barik, Rakesh; Jain, Sanjay; Qwatra, Deep; Joshi, Amit; Tripathi, Girraj Sharan; Goyal, Rav Continue reading >>

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