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Streptozotocin Nicotinamide Induced Rat Model Of Type 2 Diabetes

Streptozotocininduced Diabetic Models In Mice And Rats

Streptozotocininduced Diabetic Models In Mice And Rats

Please review our Terms and Conditions of Use and check box below to share full-text version of article. I have read and accept the Wiley Online Library Terms and Conditions of Use. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Streptozotocin (STZ) is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZinduced insulin deficiency and hyperglycemia in mice and rats. Also described are protocols for creating animal models for type 2 diabetes using STZ. These animals are employed for assessing the pathological consequences of diabetes and for screening potential therapies for the treatment of this condition. 2015 by John Wiley & Sons, Inc. Yu-Ting Tseng, Wan-Hsuan Chang, Chih-Cheng Lin, Fang-Rong Chang, Pao-Chu Wu and Yi-Ching Lo, Protective effects of Liuwei dihuang water extracts on diabetic muscle atrophy, Phytomedicine, 10.1016/j.phymed.2018.09.032, 53, (96-106), (2019). Liming Yu, Zhi Li, Xue Dong, Xiaodong Xue, Yu Liu, Shu Xu, Jian Zhang, Jinsong Han, Yang Yang and Huishan Wang, Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling, Oxidative Medicine and Cellular Longevity, 2018, (1), (2018). Haiyan Wei, Qiaoyun Hu, Junxia Wu, Chenjuan Yao, Lingfei Xu, Fengjun Xing, Xinyuan Zhao, Shali Yu, Xiaoke Wang and Gang Chen, Molecular mechanism of the increased tissue uptake of trivalent inorganic arsenic in mice with type 1 diabetes mellitus, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2018.06.029, 504, 2, (393-399), (2018). Jay Parikh, Alice Zemljic-Harpf, Johnny F Continue reading >>

Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes (review).

Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes (review).

Acta Physiol Hung. 2014 Dec;101(4):408-20. doi: 10.1556/APhysiol.101.2014.4.2. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Shahid Beheshti University of Medical Sciences Endocrine Physiology Research Center, Research Institute for Endocrine Sciences 24 Parvaneh street Velenjak, Tehran Iran PO box: 19395-4763 Shahid Beheshti University of Medical Sciences Endocrine Research Center, Research Institute for Endocrine Sciences Tehran Iran. Shahid Beheshti University of Medical Sciences Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences Tehran Iran. Diabetes is one of the five leading causes of death in the world, with type 2 diabetes occurring more frequently than type 1. Management of diabetes without side effects is still a challenge and therefore new strategies need to be examined. Because of difficulties in human research, animal models of diabetes are useful research tools for this purpose and rodent models of type 2 diabetes are the first choice. The aim of this study is an overview on one of the most frequently used models of type 2 diabetes in rat, induced by streptozotocin and nicotinamide, considering its advantages and disadvantages for diabetes research in humans. animal model; nicotinamide; rat; streptozotocin; type 2 diabetes Continue reading >>

Streptozotocin-nicotinamide-induced Diabetes In The Rat. Characteristics Of The Experimental Model.

Streptozotocin-nicotinamide-induced Diabetes In The Rat. Characteristics Of The Experimental Model.

Exp Biol Med (Maywood). 2012 May;237(5):481-90. doi: 10.1258/ebm.2012.011372. Epub 2012 May 22. Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland. [email protected] Administration of both streptozotocin (STZ) and nicotinamide (NA) has been proposed to induce experimental diabetes in the rat. STZ is well known to cause pancreatic B-cell damage, whereas NA is administered to rats to partially protect insulin-secreting cells against STZ. STZ is transported into B-cells via the glucose transporter GLUT2 and causes DNA damage leading to increased activity of poly(ADP-ribose) polymerase (PARP-1) to repair DNA. However, exaggerated activity of this enzyme results in depletion of intracellular NAD(+) and ATP, and the insulin-secreting cells undergo necrosis. The protective action of NA is due to the inhibition of PARP-1 activity. NA inhibits this enzyme, preventing depletion of NAD(+) and ATP in cells exposed to STZ. Moreover, NA serves as a precursor of NAD(+) and thereby additionally increases intracellular NAD(+) levels. The severity of diabetes in experimental rats strongly depends on the doses of STZ and NA given to these animals. Therefore, in diabetic rats, blood glucose may be changed in a broad range--from slight hyperglycemia to substantial hyperglycemia compared with control animals. Similarly, blood insulin may be only slightly decreased or substantial hypoinsulinemia may be induced. In vitro studies demonstrated that the insulin-secretory response to glucose is attenuated in STZ-NA-induced diabetic rats compared with control animals. This is due to reduced B-cell mass as well as metabolic Continue reading >>

Antidiabetic, Antihyperlipidaemic, And Antioxidant Activity Of Syzygium Densiflorum Fruits In Streptozotocin And Nicotinamide-induced Diabetic Rats

Antidiabetic, Antihyperlipidaemic, And Antioxidant Activity Of Syzygium Densiflorum Fruits In Streptozotocin And Nicotinamide-induced Diabetic Rats

Antidiabetic, antihyperlipidaemic, and antioxidant activity of Syzygium densiflorum fruits in streptozotocin and nicotinamide-induced diabetic rats Context Syzygium densiflorum Wall. ex Wight & Arn (Myrtaceae) has been traditionally used by local tribes of the Nilgiris, Tamil Nadu, India, for the treatment of diabetes, however, no definitive experimental studies are available. Objective This study investigates the antidiabetic, antihyperlipidaemic and antioxidant activities of ethanol extract of S. densiflorum (EFSD) fruits in streptozotocin (STZ) and nicotinamide (NA)-induced diabetic rats. Materials and methods Acute oral toxicity and oral glucose tolerance were assessed in normal rats. The antidiabetic, antihyperlipidaemic and antioxidant activities were investigated in STZ NA-induced diabetic rats. Diabetic rats were orally administered with glibenclamide (10 mg/kg b.wt), EFSD (200, 400 and 800 mg/kg b.wt) for 28 d. Further, changes in the blood glucose level (BGL), biochemical parameters, antioxidants were observed and histology of pancreas was performed. Results No toxicity and lethality were observed. Results of the following parameters are represented by treated versus disease control (STZ + NA) groups. BGL (161.33 22.8 versus 476.17 56.58 mg/dl), glycosylated haemoglobin (5.285 0.19 versus 8.05 0.55%), urea (40.32 1.96 versus 75.37 2.91 mg/dl), uric acid (1.2 0.07 versus 2.16 0.05 mg/dl), total cholesterol (89.3 5.14 versus 139.7 5.95 mg/dl) and triglycerides (79.65 2.52 versus 108.9 3.61 mg/dl) were significantly decreased, whereas haemoglobin (11.75 0.73 versus 7.95 0.42 g/dl), highdensity lipoprotein cholesterol (14.2 1.11 versus 6.97 0.84 mg/dl), total protein (45%) and liver glycogen (87%) were significantly increased in EFSD-treated diabetic group. Signi Continue reading >>

Hypoglycemic And Antioxidative Effects Of Glossogyne Tenuifolia On Streptozotocin-nicotinamide-induced Diabetic Rats

Hypoglycemic And Antioxidative Effects Of Glossogyne Tenuifolia On Streptozotocin-nicotinamide-induced Diabetic Rats

Hypoglycemic and Antioxidative Effects of Glossogyne tenuifolia on Streptozotocin-Nicotinamide-Induced Diabetic Rats Download as PDF (Size:632KB) PP. 1170-1181 DOI: 10.4236/ajps.2017.85077 588 Downloads 959 Views Citations Glossogyne tenuifolia (GT) is the traditional herbal tea in Penghu Island, Taiwan. Recent research hasshown that the active components in GT are potential inhibitors of -glucosidase.The present study investigated that whether or not GT could improve the statusof type 2 diabetes mellitus. Male Wistar rats aged eight weeks were induced tobe hyperglycemic by subcutaneous injection of streptozotocin-nicotinamide (STZ-NA) andcombination of high-fat diet (HFD). The animals were given GT extractsat the low dose or high dose, or the anti-diabetic drug (acarbose), in drinkingwater for 4 weeks. The results showed that hot water extracts from GT resultedin significantly decreases in fasting blood glucose at the 1st and 2nd weeks,fasting insulin levels at the 2nd week, 1 hour postprandial blood glucose after the starch tolerance test on Day 23 and blood glucose levels after oral glucose tolerancetest (OGTT) at the 60th minute on Day 25. In addition, diabetic rats treated with GT extractsfrom hot water for 4 weeks displayed significantly decreased thiobarbituricacid reactive substances (TBARS) in the serum, liver and kidney, serum totalcholesterol, fasting insulin levels and homeostasis model assessment forinsulin resistance (HOMA-IR). Overall, these resultsdemonstrate that the hot waterextracts of GT might improve the progression of diabetes and decrease oxidativestress in STZ-NA-induceddiabetic rats. Glossogyne tenuifolia , Blood Glucose , Antioxidative , Diabetic Rats Diabetes mellitus (DM) is currently recognized as a chronic metabolic disease with the highes Continue reading >>

Reversal Of Endothelial Dysfunction In Aorta Of Streptozotocin-nicotinamide-induced Type-2 Diabetic Rats By S-allylcysteine

Reversal Of Endothelial Dysfunction In Aorta Of Streptozotocin-nicotinamide-induced Type-2 Diabetic Rats By S-allylcysteine

, Volume 432, Issue12 , pp 2532 | Cite as Reversal of endothelial dysfunction in aorta of streptozotocin-nicotinamide-induced type-2 diabetic rats by S-Allylcysteine Dietary measures and plantbased therapies as prescribed by native systems of medicine have gained attraction among diabetics with claims of efficacy. The present study investigated the effects of S-Allylcysteine (SAC) on body weight gain, glucose, insulin, insulin resistance, and nitric oxide synthase in plasma and argininosuccinate synthase (AS) and argininosuccinate lyase (ASL), lipid peroxides and antioxidant enzymes in aorta of control and streptozotocin-nicotinamide (STZ-NA)-induced diabetic rats. Changes in body weight, glucose, insulin, insulin resistance, and antioxidant profiles of aorta and mRNA expressions of nitric oxide synthase, AS, and ASL were observed in experimental rats. SAC (150mg/kg b.w) showed its therapeutic effects similar to gliclazide in decreasing glucose, insulin resistance, lipid peroxidation, and increasing body weight; insulin, antioxidant enzymes, and mRNA levels of nitric oxide synthase, argininosuccinate synthase, and argininosuccinate lyase genes in STZ-NA rats. Histopathologic studies also revealed the protective nature of SAC on aorta. In conclusion, garlic and its constituents mediate the anti-diabetic potential through mitigating hyperglycemic status, changing insulin resistance by alleviating endothelial dysregulation in both plasma and tissues. S-AllylcysteineDiabetesDiabetic cardiomyopathyLipid peroxidationAortaStreptozotocin Parim Brahmanaidu and V. V. Sathibabu Uddandrao have contributed equally to this work. This is a preview of subscription content, log in to check access The authors thank the Department of Science and Technology, India, for providing financial Continue reading >>

Effect Of Atorvastatin On Pharmacology Of Sitagliptin In Streptozotocin-nicotinamide Induced Type-ii Diabetes In Rats

Effect Of Atorvastatin On Pharmacology Of Sitagliptin In Streptozotocin-nicotinamide Induced Type-ii Diabetes In Rats

Received date: December 17, 2014; Accepted date: December 31, 2014; Published date: January 07, 2015 Citation: Eggadi V, Sheshagiri SBB, Devandla A, Dasi N, Kulundaivelu U, et al. (2015) Effect of Atorvastatin on Pharmacology of Sitagliptin in Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats. Biol Med 7:225. doi:10.4172/0974-8369.1000225 Copyright: 2015 Eggadi V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Prolonged type 2 diabetes mellitus (Type2DM) may lead to high risk of cardiovascular disease (CVD) requiring a global therapeutic approach. Statin therapy has proven its efficacy in reducing CVD events in Type2 DM patients. Dipeptidyl peptidase-4 inhibitors (gliptins), which are increasingly used to target hyperglycemia. In the present study type 2 DM in rats by i.p. Administration of Streptozotocin (STZ); (60 mg/kg) -Nicotinamide (120 mg/kg). Diabetes induced rats were divided into groups and treated with Sitagliptin alone and in combination with atorvastatin for 7 days. Blood samples were collected by retro orbital puncture. Mean glucose concentration was measured by GODPOD method using commercial glucose kits and sitagliptin in plasma was estimated by RP-HPLC method using methanol: water (60:40 v/v, containing 10 mM Tris and 10 mM Triethylamine) was adjusted to pH 9.0 using 1 mol/L hydrochloric acid. The blood glucose lowering activity of sitagliptin was increased by the presence of atorvastatin in diabetic rats. The pharmacokinetic (PK) and pharmacodynamic (PD) parameters of sitagliptin in diabetic rats were significantly changed in the presence of atorvas Continue reading >>

Magnesium Upregulates Insulin Receptor And Glucose Transporter-4 In Streptozotocin-nicotinamide-induced Type-2 Diabetic Rats

Magnesium Upregulates Insulin Receptor And Glucose Transporter-4 In Streptozotocin-nicotinamide-induced Type-2 Diabetic Rats

Magnesium upregulates insulin receptor and glucose transporter-4 in streptozotocin-nicotinamide-induced type-2 diabetic rats Magnesium upregulates insulin receptor and glucose transporter-4 in streptozotocin-nicotinamide-induced type-2 diabetic rats Department of Biochemistry, College of Medicine, University of Lagos, Nigeria Department of Physiology, Benjamen S. Carson (Snr) School of Medicine, Babcock University, Babcock, Nigeria Published Online: 2018-02-16 | DOI: Objective. We investigated the effects of magnesium supplementation on glucose tolerance, insulin sensitivity, oxidative stress as well as the concentration of insulin receptor and glucose transporter-4 in streptozotocin-nicotinamide induced type-2 diabetic (T2D) rats. Methods. Rats were divided into four groups designated as: 1) control (CTR); 2) diabetic untreated (DU); 3) diabetic treated with 1 mg of Mg/kg diet (Mg1-D); and 4) diabetic treated with 2 mg of Mg/kg diet (Mg2-D). T2D was induced with a single intraperitoneal (i.p.) injection of freshly prepared streptozotocin (55 mg/kg) aft er an initial i.p. injection of nicotinamide (120 mg/kg). Glucose tolerance, insulin sensitivity, lipid profile, malondialdehyde (MAD) and glutathione content, insulin receptors (INSR) and glucose transporter-4 (GLUT4), fasting insulin and glucose levels were measured, and insulin resistance index was calculated using the homeostatic model assessment of insulin resistance (HOMA-IR). Results. Magnesium supplementation improved glucose tolerance and lowered blood glucose levels almost to the normal range. We also recorded a noticeable increase in insulin sensitivity in Mg-D groups when compared with DU rats. Lipid perturbations associated T2D were significantly attenuated by magnesium supplementation. Fasting glucose leve Continue reading >>

Non-genetic Rat Model Of Nephropathy In Type 2 Diabetes With Attenuated Streptozotocin-induced Tubular Alteration

Non-genetic Rat Model Of Nephropathy In Type 2 Diabetes With Attenuated Streptozotocin-induced Tubular Alteration

Endocrine Abstracts (2017) 49 GP92 | DOI: 10.1530/endoabs.49.GP92 Non-genetic rat model of nephropathy in type 2 diabetes with attenuated streptozotocin-induced tubular alteration Valentina Bayrasheva1,2, Alina Babenko1,2, Ivan Pchelin3, Anna Arefjeva2, Svetlana Chefu1,2, Ivan Shatalov4, Yurii Dmitriev1, Alexandra Ivanova3,5, Pavel Andoskin1, Parvis Aliev2 & Elena Grineva1,2 1Federal Almazov North-West Medical Research Centre, Saint Petersburg, Russia; 2Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia; 3Saint Petersburg State University, Saint Petersburg, Russia; 4Saint Petersburg National Research University of Information, Technologies, Mechanics and Optics, Saint Petersburg, Russia; 5Komarov Botanical Institute of the Russian Academy of Sciences, Saint Petersburg, Russia. Non-genetic animal models of diabetic nephropathy (DN) are most commonly reproduced by using streptozotocin (STZ) which preferentially gets into -cells via GLUT2 transporters. However, STZ administration results in nephrotoxic effects as well, due to expression of GLUT2 by renal tubular epithelial cells. We hypothesized that nicotinamide (NA), which is considered to attenuate the severity of STZ-induced -cell damage, could also prevent tubular alteration. Starting at 3 weeks after unilateral nephrectomy, thirty adult male Wistar rats were fed the high-fat diet for 5 weeks and then successively received either NA (230 mg/kg) and STZ (65 mg/kg, NA-STZ-group) or STZ in a low dose (40 mg/kg, LD-STZ-group) intraperitoneally in 15-min interval. Control nondiabetic uninephrectomized rats received vehicle and were fed normal chow (C-group). At weeks 10, 20, and 30 (the end of the study), metabolic parameters, creatinine clearance, albuminuria, and urinary tubular injury mark Continue reading >>

Eugenosedin-a Improves Glucose Metabolism And Inhibits Mapks Expression In Streptozotocin/nicotinamide-induced Diabetic Rats - Sciencedirect

Eugenosedin-a Improves Glucose Metabolism And Inhibits Mapks Expression In Streptozotocin/nicotinamide-induced Diabetic Rats - Sciencedirect

Eugenosedin-A improves glucose metabolism and inhibits MAPKs expression in streptozotocin/nicotinamide-induced diabetic rats Author links open overlay panel Kuo-PingShena Hui-LiLinb Hsueh-WeiYenc Su-LingHsiehd Li-MeiAne Bin-NanWuefg Open Access funded by Kaohsiung Medical University This study examined the effects of eugenosedin-A (Eu-A) in a streptozotocin (STZ)/nicotinamide-induced rat model of type II diabetes mellitus (T2DM). Six-week-old SpragueDawley rats were randomly divided into three groups: (1) RD group, normal rats fed a regular diet (RD), (2) DM group, T2DM rats fed a high-fat diet, and (3) Eu-A group, T2DM rats fed a high fat diet plus oral Eu-A (5mg/kg/day). After 30 days, the DM group had higher body weight, higher blood glucose and lower insulin levels than the RD group. The DM group also had increased protein expression of glycogen synthase kinase (GSK) in liver and skeletal muscle and decreased protein expression of insulin receptor (IR), insulin receptor substrate-1 (IRS-1), IRS-2, AMP-activated protein kinase (AMPK), glucose transporter-4 (GLUT-4), glucokinase (GCK), and peroxisome proliferator-activated receptor (PPAR-). STZ/nicotinamide-induced T2DM increased the expression of mitogen-activated protein kinases (MAPKs: p38, ERK, JNK) and inflammatory p65 protein. In the Eu-A treated T2DM rats, however, blood glucose was attenuated and the insulin concentration stimulated. Changes in IR, IRS-1 and IRS-2 proteins as well as AMPK, GLUT-4, GCK, GSK, PPAR-, MAPKs, and inflammatory p65 proteins were ameliorated. These results suggested that Eu-A alleviates STZ/nicotinamide-induced hyperglycemia by improving insulin levels and glucose metabolism, and inhibiting the MAPKs- and p65-mediated inflammatory pathway. Continue reading >>

High Fat Diet/stz Induced Diabetic Model

High Fat Diet/stz Induced Diabetic Model

Home / Lead Optimization / Pharmacology and Pharmacodynamics Studies / Rodent Models / Non-Genetically Engineering Models (NON-GEMs) / Rodent Metabolic Disease Models / High Fat Diet/STZ Induced Diabetic Model Creative Biolabs has extensive experience in establishing chemically induced diabetic mice/rats models for our global customers, especially for the high fat diet/STZ induced diabetic model. Type 2 diabetes is a long-term metabolic disorder characterized by high blood sugar, insulin resistance and relative lack of insulin. Now there has been a tragic increase in diabetes worldwide, paralleling the overweight and obesity epidemic. In order to further elucidate the pathobiology and to find better treatments and novel prevention strategies for this complex disease, appropriate experimental animal models are of great value. One example of type 2 diabetes animal models is the high fat diet-fed, streptozotocin (HFD/STZ)-treated rat model. Rats fed with high-fat diet are useful models to mimic insulin resistance, which is one of the important features of type 2 diabetes. At the same time, high-dose streptozotocin (STZ) is widely used to induce type 1 diabetes in adult mice, resulting from the cell death through alkylation of DNA. However, low-dose STZ has been shown to induce a mild impairment of insulin secretion, which is similar to the feature of the later stage of type 2 diabetes. Based on the above two points, investigators have started to develop a new diabetic model by the combination of diet (high fat or high fructose diet) plus the treatment of STZ. In the first place, rats are fed with above special diets, which initially produces hyperinsulinemia and insulin resistance. Subsequently, these sensitive subjects are treated with low-dose STZ that causes the cell d Continue reading >>

Prime Pubmed | Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes (review

Prime Pubmed | Streptozotocin-nicotinamide-induced Rat Model Of Type 2 Diabetes (review

Type your tag names separated by a space and hit enter Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Diabetes is one of the five leading causes of death in the world, with type 2 diabetes occurring more frequently than type 1. Management of diabetes without side effects is still a challenge and therefore new strategies need to be examined. Because of difficulties in human research, animal models of diabetes are useful research tools for this purpose and rodent models of type 2 diabetes are the first choice. The aim of this study is an overview on one of the most frequently used models of type 2 diabetes in rat, induced by streptozotocin and nicotinamide, considering its advantages and disadvantages for diabetes research in humans. Ghasemi A, Khalifi S, Jedi S: "Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review)." Acta physiologica Hungarica, vol. 101, no. 4, 2014, pp. 408-20, Accessed November 29, 2018. Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta Physiol Hung 2014;101(4):408-20 Accessed November 29, 2018. Ghasemi A & Khalifi S & Jedi S. (2014). Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta physiologica Hungarica, 101, pp. 408-20. doi:10.1556/APhysiol.101.2014.4.2 Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta Physiol Hung. 2014;101:408-20 TY - JOURT1 - Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review).AU - Ghasemi,Asghar,AU - Khalifi,S,AU - Jedi,S,PY - 2014/12/24/entrezPY - 2014/12/24/pubmedPY - 2015/3/12/medlineKW - animal modelKW - nicotinamideKW - ratKW - streptozotocinKW - type 2 diabetesSP - 408EP - 20JF - Acta physiologica Hunga Continue reading >>

The Hypoglycemic Activity Of Euclea Undulata Thunb. Var. Myrtina (ebenaceae) Root Bark Evaluated In A Streptozotocin-nicotinamide Induced Type 2 Diabetes Rat Model

The Hypoglycemic Activity Of Euclea Undulata Thunb. Var. Myrtina (ebenaceae) Root Bark Evaluated In A Streptozotocin-nicotinamide Induced Type 2 Diabetes Rat Model

The hypoglycemic activity of Euclea undulata Thunb. var. myrtina (Ebenaceae) root bark evaluated in a streptozotocin-nicotinamide induced type 2 diabetes rat model JavaScript is disabled for your browser. Some features of this site may not work without it. The hypoglycemic activity of Euclea undulata Thunb. var. myrtina (Ebenaceae) root bark evaluated in a streptozotocin-nicotinamide induced type 2 diabetes rat model Deutschlander, M.S. (Miranda Susan); Lall, Namrita; Van de Venter, Maryna; Dewanjee, S. The hypoglycaemic activity of a crude acetone extract of the root bark of Euclea undulata var. myrtina was evaluated in a streptozotocin-nicotinamide induced type 2 diabetes rat model after positive results were obtained by in vitro screening of glucose utilization by C2C12 myocytes, 3T3-L1 preadipocytes and Chang liver cells and alpha-glucosidase inhibition. Thirty male Wistar rats were used for the experiment. Type 2 diabetes was induced by a single intraperitoneal injection of streptozotocin and administration of nicotinamide 15 minutes after. Animals exhibiting fasting glucose levels of 140-200 mg/dl after 7 days were screened as type 2 diabetes. Extract was administered for 21 days orally at a concentration of 50 mg/kg and 100 mg/kg respectively. Glibenclamide (1mg/kg) was used as positive control. On day 21, blood lipid profiles and body weight were determined by using standard enzymatic colorimetric kits before the rats were sacrificed by cervical decapitation. The crude acetone extract of E. undulata root bark at a concentration of 100mg/kg body weight significantly lowered fasting blood glucose levels as well as elevated cholesterol and triglyceride levels to near normal without any weight gain. The results indicate that the crude acetone root bark extract of E Continue reading >>

Streptozotocininduced Diabetic Models In Mice And Rats

Streptozotocininduced Diabetic Models In Mice And Rats

Please review our Terms and Conditions of Use and check box below to share full-text version of article. I have read and accept the Wiley Online Library Terms and Conditions of Use. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Streptozotocin (STZ) is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZinduced insulin deficiency and hyperglycemia in mice and rats. Also described are protocols for creating animal models for type 2 diabetes using STZ. These animals are employed for assessing the pathological consequences of diabetes and for screening potential therapies for the treatment of this condition. 2015 by John Wiley & Sons, Inc. Yu-Ting Tseng, Wan-Hsuan Chang, Chih-Cheng Lin, Fang-Rong Chang, Pao-Chu Wu and Yi-Ching Lo, Protective effects of Liuwei dihuang water extracts on diabetic muscle atrophy, Phytomedicine, 10.1016/j.phymed.2018.09.032, 53, (96-106), (2019). Liming Yu, Zhi Li, Xue Dong, Xiaodong Xue, Yu Liu, Shu Xu, Jian Zhang, Jinsong Han, Yang Yang and Huishan Wang, Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling, Oxidative Medicine and Cellular Longevity, 2018, (1), (2018). Haiyan Wei, Qiaoyun Hu, Junxia Wu, Chenjuan Yao, Lingfei Xu, Fengjun Xing, Xinyuan Zhao, Shali Yu, Xiaoke Wang and Gang Chen, Molecular mechanism of the increased tissue uptake of trivalent inorganic arsenic in mice with type 1 diabetes mellitus, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2018.06.029, 504, 2, (393-399), (2018). Jay Parikh, Alice Zemljic-Harpf, Johnny F Continue reading >>

Streptozotocin-nicotinamide Induced Diabetic: Topics By Science.gov

Streptozotocin-nicotinamide Induced Diabetic: Topics By Science.gov

Barik, Rakesh; Jain, Sanjay; Qwatra, Deep; Joshi, Amit; Tripathi, Girraj Sharan; Goyal, Ravi Objective: To evaluate the antidiabetic activity of aqueous extract of roots of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats. Materials and Methods: Streptozotocin-nicotinamide induced type-II diabetic rats (n = 6) were administered aqueous root extract (250 and 500 mg/kg, p.o.) of Ichnocarpus frutescens or vehicle (gum acacia solution) or standard drug glibenclamide (0.25 mg/kg) for 15 days. Blood samples were collected by retro-orbital puncture and were analyzed for serum glucose on days 0, 5, 10, and 15 by using glucose oxidase-peroxidase reactive strips and a glucometer. For oral glucose tolerance test, glucose (2 g/kg, p.o.) was administered to nondiabetic control rats and the rats treated with glibenclamide (10 mg/kg, p.o.) and aqueous root extract of Ichnocarpus frutescens. The serum glucose levels were analyzed at 0, 30, 60, and 120 min after drug administration. The effect of the extract on the body weight of the diabetic rats was also observed. Results: The aqueous root extract of Ichnocarpus frutescens (250 and 500 mg/kg, p.o.) induced significant reduction (P < 0.05) of fasting blood glucose levels in streptozotocin-nicotinamide induced type-II diabetic rats on the 10th and 15th days. In the oral glucose tolerance test, the extract increased the glucose tolerance. It also brought about an increase in the body weight of diabetic rats. Conclusion: It is concluded that Ichnocarpus frutescens has significant antidiabetic activity as it lowers the fasting blood sugar level in diabetic rats and increases the glucose tolerance. PMID:21264156 Barik, Rakesh; Jain, Sanjay; Qwatra, Deep; Joshi, Amit; Tripathi, Girraj Sharan; Goyal, Rav Continue reading >>

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