Leptin Levels In Children With Type 1 Diabetes Mellitus.
Leptin Levels in Children with Type 1 Diabetes Mellitus. Journal of Korean Society of Pediatric Endocrinology 2000;5(1): 93-99. Leptin Levels in Children with Type 1 Diabetes Mellitus. Department of Pediatrics, Seoul National University Children`s Hospital, Seoul, Korea. Leptin is a product of ob gene in adipose tissue.This is a important factor for regulation of body weight, especially body fat mass. So it is interested in correlation of leptin with obesity or various diseases related with obesity, particularly diabetes mellitus. It was demonstrated that leptin level was increased in type 2 diabetes and insulin was associated with increased leptin level. This study is to measure leptin levels in type 1 diabetes without insulin resistance, to validate whether insulin increases leptin level and to investigate correlation of leptin with degree of diabetic control and pubertal progression.METHODS: This study included 114 children(boys; 57, girls; 57) with type 1 diabetes, whose blood samples were taken to measure leptin level using RIA(radioimmunoassay). We observed the correlation of leptin with body mass index, HbA1c, insulin dose, duration of disease, and pubertal degree. Mean values of age, BMI, insulin dose, and HbA1c at the measurement of leptin were 13.0+/-5.04 years-old, 18.9+/-3.5kg/m2, 36U/day, and 8.9+/-2.1%, respectively.Thirty seven children were prepubertal and fifty seven children had pubertal characteristics and the rests were not confirmed. With increment of BMI, serum leptin levels were significantly increased in the all subjects, with the same correlation in pubertal female group, but not in pubertal male. There was a relatively weak correlation(r=0.29) of leptin levels with insulin doses. In prepubertal male, there was a significant positive correlatio Continue reading >>
Leptin Resistance: The Other Hormone Important For Managing Diabetes | Everyday Health
Eating a Mediterranean-style diet with lean protein, whole grains, and healthy fats can help increase leptin sensitivity. When most people think of diabetes and hormones, insulin which controls blood sugar levels by ferrying glucose to cells for energy is usually the first that springs to mind. But there are many hormones at play when it comes to blood sugar levels and weight, and anyone living with diabetes or prediabetes should consider them all when striving to better manage their symptoms. Key among these is leptin, a hormone produced by fat cells that helps the body regulate energy by telling the brain its had enough to eat, according to a review published in December 2012 in the Indian Journal of Endocrinology and Metabolism . Leptins action is to inhibit appetite, to stimulate the burning up of fatty acids, to decrease glucose, and to reduce overall body fat and weight, says VandanaSheth, RDN, CDE , a spokesperson for the Academy of Nutrition and Dietetics. The problem, Sheth explains, is, just like insulin resistance whereby cells are resistant to insulin, leading glucose to accumulate in the blood people can develop leptin resistance, an effect that can further complicate their diabetes management . When this occurs, Sheth says, People can gain weight, have increased body fat, and, even though there are adequate fat stores, their brains are getting the signal that they are hungry, so they eat more. It's a vicious cycle. Unfortunately, just adding more leptin, as people with diabetes often do with insulin injections , cant fix this problem because the issue isnt always how much leptin is in the body, but whether the body is able to process it efficiently. In fact, for people without diabetes, or for those who have been diagnosed with prediabetes, leptin resista Continue reading >>
Insulin-stimulated Increase In Serum Leptin Levels Precedes And Correlates With Weight Gain During Insulin Therapy In Type 2 Diabetes
The Journal of Clinical Endocrinology & Metabolism Insulin-Stimulated Increase in Serum Leptin Levels Precedes and Correlates with Weight Gain during Insulin Therapy in Type 2 Diabetes Departments of Nutrition and Dietetics (A.-M.A.) Aker University Hospital, 0514 Oslo, Norway University of Oslo, Faculty Division Aker (A.-M.A., K.F.H., J.P.B., P.M.T., K.I.B.), Aker University Hospital, 0514 Oslo, Norway Address all correspondence and requests for reprints to: Anne-Marie Aas, Department of Nutrition and Dietetics, Aker University Hospital, Trondheimsveien 235, 0514 Oslo, Norway. Search for other works by this author on: Endocrinology (K.F.H., J.P.B., P.M.T., K.I.B.) Aker University Hospital, 0514 Oslo, Norway University of Oslo, Faculty Division Aker (A.-M.A., K.F.H., J.P.B., P.M.T., K.I.B.), Aker University Hospital, 0514 Oslo, Norway Search for other works by this author on: Endocrinology (K.F.H., J.P.B., P.M.T., K.I.B.) Aker University Hospital, 0514 Oslo, Norway University of Oslo, Faculty Division Aker (A.-M.A., K.F.H., J.P.B., P.M.T., K.I.B.), Aker University Hospital, 0514 Oslo, Norway Search for other works by this author on: Endocrinology (K.F.H., J.P.B., P.M.T., K.I.B.) Aker University Hospital, 0514 Oslo, Norway University of Oslo, Faculty Division Aker (A.-M.A., K.F.H., J.P.B., P.M.T., K.I.B.), Aker University Hospital, 0514 Oslo, Norway Search for other works by this author on: Endocrinology (K.F.H., J.P.B., P.M.T., K.I.B.) Aker University Hospital, 0514 Oslo, Norway University of Oslo, Faculty Division Aker (A.-M.A., K.F.H., J.P.B., P.M.T., K.I.B.), Aker University Hospital, 0514 Oslo, Norway Search for other works by this author on: The Journal of Clinical Endocrinology & Metabolism, Volume 94, Issue 8, 1 August 2009, Pages 29002906, Anne-Marie Aas, Krist Continue reading >>
Serum Leptin Levels In Type 2 Diabetic Pakistani Subjects And Its Correlation With Fasting Blood Sugar Rafique N, Latif R - Saudi J Health Sci
Background: Most of the data documenting serum leptin levels in diabetes comes from Caucasians and a very limited data is available pertaining to non-Caucasian subjects. The aim of this study was to determine serum leptin levels in Pakistani subjects and its correlation with fasting blood sugar (FBS) among normal and type 2 diabetic adults. Settings and Design: It was a case-control study including type 2 diabetic patients having FBS >126 mg/dl. Exclusion criteria included the subjects from whom the written consent could not be taken, the patients with acute inflammatory disease in the last 15 days (as acute inflammation may lead to hyperleptinemia) or FBS between 101 and 126 mg/dl. Control group consisted of nondiabetic subjects when FBS was <101 mg/dl. Materials and Methods: An observational study was carried out at Shifa College of Medicine, Islamabad. Serum leptin by sandwich enzyme-linked immunosorbent assay (ELISA) method, FBS by glucometer were measured in 64 type 2 diabetic patients and compared with their age-, sex-, and body mass index ( BMI)-matched nondiabetic controls. Statistical Analysis Used: Independent t-test was used to find out whether there was any statistical significance in between the two groups. Statistical significance was set at the 95% confidence level (P < 0.05). Pearson's correlation was used to find out the relation between serum leptin and FBS in diabetics. Results: Serum leptin levels were significantly (P < 0.05) high in diabetics when compared with the control group. A significant positive correlation was found between serum leptin and FBS. Conclusions: On the basis of our results, we conclude that type 2 diabetic Pakistani subjects have high serum leptin which correlates positively with FBS. Keywords:Correlation, fasting blood sugar, Continue reading >>
Leptin And Levels And Risk Of Type 2 Diabetes In Women
ObjectiveWe prospectively examined plasma levels of leptin and soluble leptin receptor (sOB-R), as well as their interactions with other diabetes risk factors, in relation to type 2 diabetes to elucidate the complex relation between these two biomarkers and diabetes risk. Research Design and MethodsOf 32,826 Nurses' Health Study participants who provided blood samples during 19891990, 1,054 incident case subjects of type 2 diabetes were identified and confirmed during 19902004 and 1,254 matched control subjects were selected. Plasma leptin and sOB-R levels were measured among these participants. ResultsAfter multivariate adjustment for BMI, lifestyle practices, and dietary factors, sOB-R levels were significantly associated with a lower risk of type 2 diabetes. In comparison with women in the lowest quintile, the ORs (95% CI) of developing type 2 diabetes were 0.73 (0.550.96), 0.51 (0.380.68), 0.42 (0.310.57), and 0.39 (0.280.54; P for trend < 0.0001) for women in the second to fifth quintiles of sOB-R levels, respectively. In contrast, plasma leptin levels were not significantly associated with the risk of type 2 diabetes: The OR (95% CI) was 0.82 (0.621.10; P for trend = 0.46) comparing the highest with the lowest quintile of leptin levels. sOB-R levels were consistently associated with a decreased risk of type 2 diabetes at various levels of leptin or high-molecular-weight adiponectin. ConclusionsThese data suggest a strong inverse association between plasma sOB-R levels and risk of type 2 diabetes, independent of BMI, leptin, and adiponectin levels. Leptin, a 16-kDa protein produced primarily in adipose tissue,[ 1 ] is a pleiotropic hormone that is involved in body weight regulation, puberty, reproduction, and immune function.[ 24 ] Although accumulating evidence s Continue reading >>
Leptin And Diabetes Are You Leptin Resistant?
Leptin and Diabetes Are You Leptin Resistant? We know insulin resistance contributes to Type 2 diabetes. But resistance to another hormone called leptin also contributes big time. Fortunately, we may be able to turn down both kinds of resistance. Leptin helps regulate body fat, in part by telling the brain what level of fat is in the body and how that level is changing. When fat stores are sufficient, it lets the brain know, Were full. We have enough food now. So stop eating. Some Indian scientists put it in more technical terms : The net action of leptin is to inhibit appetite, stimulate thermogenesis [heat production], [burn up fatty acids], decrease glucose, and reduce body weight and fat. Those are good things for most of us. We want that. But many people, especially fat people, are leptin resistant. Talking to the UK newspaper Daily Mail, anti-sugar crusader Robert Lustig, MD, said, This means thebrainno longer reads the signals saying the body is full, but instead assumes it is starving no matter how much food you continue to eat. So leptin resistance (LR) contributes to fatness in much the same way that insulin resistance (IR) contributes to Type 2 diabetes. In fact, the two frequently go together. Not always as we know, most fat people dont have diabetes, and many people with Type 2 are thin. But a huge number of people have both types of resistance, which is why fat people are more likely to get diabetes, and diabetes gets wrongly blamed on fat. In fact, Dr. Lustig believes insulin resistance triggers LR . Insulin blocks leptins fullness signals in the brain. His studies have found that lowering insulin levels stops cravings and helps people get control of their food consumption, presumably because leptin is working again. Scientists used to think increasing l Continue reading >>
Association Of Plasma Leptin Levels With Type 2 Diabetes In Rotating Shift Work Women
Research Article - Biomedical Research (2017) Volume 28, Issue 1 Association of plasma leptin levels with type 2 diabetes in rotating shift work women Shaobo Wang 1 * , Wenjin Fu 2 , Youming Jiang 2 , Li Deng 1 , Shancun Zhou 1 and Lei Yang 1 1 Department of Endocrinology, Affiliated Houjie Hospital, Guangdong Medical College, Guangdong, PR China 2 Department of Laboratory, Affiliated Houjie Hospital, Guangdong Medical College, Guangdong, PR China Department of endocrinology,Affiliated Houjie Hospital Visit for more related articles at Biomedical Research Objective: We aimed to study the association among leptin levels, sleep duration, PSQI and risks of type 2 diabetes in Chinese women nurses. Methods: We conducted a cross sectional study, 65 diabetics and age matched 72 non-diabetic group were assessed for physical and chemical parameters like Body Mass Index, PSQI, leptin levels, insulin levels and fasting blood glucose. Plasma leptin level was measured by direct sandwich ELISA. Results: Plasma leptin levels were significantly correlated with age, insulin level, BMI, but not correlated with duration of shift work, sleep duration and PSQI score. Logistic regression analysis showed that a negative association between leptin levels and incidence of diabetes adjusted by age, BMI, sleep duration, PSQI score, duration of shift work and insulin. The odd ratio for diabetes being 0.868 (95% CI 0.597-1.711), 0.793 (95% CI 0.448-0.792), for 2nd tertile and 3rd tertile, respectively. However, we have not found the significant association of sleep duration, PSQI score with diabetes. Conclusion: An independent negative association was found between leptin levels and the incidence of type 2 diabetes in Chinese women with rotating shift work. However, there were no correlation of sl Continue reading >>
Leptin And Incident Type 2 Diabetes: Risk Or Protection?
, Volume 49, Issue9 , pp 20862096 | Cite as Leptin and incident type 2 diabetes: risk or protection? The aim of this study was to investigate the association of leptin levels with incident diabetes in middle-aged adults, taking into account factors purportedly related to leptin resistance. We conducted a casecohort study (570 incident diabetes cases and 530 non-cases) representing the 9-year experience of 10,275 participants of the Atherosclerosis Risk in Communities Study. Plasma leptin was measured by direct sandwich ELISA. In proportional hazards models adjusting for age, study centre, ethnicity and sex, high leptin levels (defined by sex-specific cut-off points) predicted an increased risk of diabetes, with a hazard ratio (HR) comparing the upper with the lower quartile of 3.9 (95% CI 2.65.6). However, after further adjusting additionally for obesity indices, fasting insulin, inflammation score, hypertension, triglycerides and adiponectin, high leptin predicted a lower diabetes risk (HR=0.40, 95% CI 0.230.67). Additional inclusion of fasting glucose attenuated this protective association (HR=0.59, 95% CI 0.321.08, p<0.03 for linear trend across quartiles). In similar models, protective associations were generally seen across subgroups of sex, race, nutritional status and smoking, though not among those with lower inflammation scores or impaired fasting glucose (interaction p=0.03 for both). High leptin levels, probably reflecting leptin resistance, predict an increased risk of diabetes. Adjusting for factors purportedly related to leptin resistance unveils a protective association, independent of adiponectin and consistent with some of leptins described protective effects against diabetes. Inflammatory markersLeptinLeptin resistanceRisk factorsType 2 diabetes Diabe Continue reading >>
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Leptin Levels In Type 2 Diabetes: Associations With Measures Of Insulin Resistance And Insulin Secretion.
Leptin levels in type 2 diabetes: associations with measures of insulin resistance and insulin secretion. Department of Diabetology, Metabolism and Clinical Nutrition, University Hospital Antwerp, Belgium. Interactions between leptin and insulin have been shown previously, in vitro and in vivo. In this study, we evaluate the associations of leptin levels with insulin secretion and insulin sensitivity in type 2 diabetes. Fasting leptin levels, HbA 1c, glucose, insulin, C-peptide, intact and des-31,32-proinsulin were measured in 100 non-insulin-treated type 2 diabetic patients. Glucose, insulin and C-peptide were measured 2 hours after an oral glucose load. Insulin resistance and beta-cell function were calculated using HOMA. Leptin levels were found to be associated with all measures of beta-cell secretion: with fasting and 2 hours insulin and C-peptide, with intact and des-31,32-proinsulin concentrations, and with beta-cell secretion estimated with HOMA. This association was independent of age and body fat in women, but in men, associations with insulin and C-peptide weakened after controlling for fat mass, whereas those with intact and des-31,32-proinsulin disappeared. Fasting insulin and C-peptide levels were also significant in multiple regression analyses, besides gender and fat mass. Insulin resistance, as assessed by HOMA, was strongly correlated with leptin, also after correction for age and fat mass in both genders. We conclude that, besides fat mass and gender - the main determinants for leptin levels in type 2 diabetic subjects as in healthy subjects - insulin secretion and the degree of insulin resistance also seem to contribute significantly to leptin levels. Continue reading >>
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A Review Of The Leptin Hormone And The Association With Obesity And Diabetes Mellitus
Received Date: February 06, 2017; Accepted Date: March 01, 2017; Published Date: March 08, 2017 Citation: Facey A, Dilworth L, Irving R (2017) A Review of the Leptin Hormone and the Association with Obesity and Diabetes Mellitus. JDiabetes Metab 8:727. doi:10.4172/2155-6156.1000727 Copyright: 2017 Facey A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Leptin is a protein hormone that regulates food intake. It is secreted by the adipocytes and contains 167 amino acids. The hormone signals the hypothalamus and is released to reduce the desire for food thereby controlling appetite. Research shows that diet-induced obesity results in leptin resistance, so while the level of leptin may be high, it is ineffective. Leptin is also increased in type 2 diabetes mellitus which is strongly associated with obesity and insulin resistance . While leptin may be elevated in both diabetes and obesity, it is not the cause of either disease. It has been suggested that leptin mediates insulin resistance and as such may be a possible treatment for insulin resistance. Diet induced leptin resistance can be reversed through dietary changes and its administration can be effective in combating insulin resistance. Leptin levels may also decrease with increased exercise. The pathophysiology involving leptin resistance is unclear. More leptin studies are needed as it may be an unexplored treatment for diabetes and may be able to provide a more detailed understanding of the disease. Leptin; Obesity; Diabetes; Insulin resistance Leptin which contains 167 amino acids, was discovered in 1994. It isa hormone s Continue reading >>
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Evaluation Of Leptin As A Marker Of Insulin Resistance In Type 2 Diabetes Mellitus Moonishaa Tm, Nanda Sk, Shamraj M, Sivaa R, Sivakumar P, Ravichandran K - Int J App Basic Med Res
Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia over a prolonged period.  As of 2014, an estimated 387 million people have diabetes worldwide, of which type 2 DM (T2DM) makes up about 90% constituting about 8.3% of the total adult population.  Insulin resistance (IR) is a condition in which cells fail to respond to the normal actions of the hormone insulin, despite its normal secretion. The resultant effects are impaired glucose utilization by the cells, leading to hyperglycemia. IR along with decreased insulin secretion results in T2DM.  Homeostatic Model Assessment (HOMA) is a method used to quantify IR.  Leptin is an adipocyte-derived hormone, which is also known as an adipocytokine as it plays a role in the inflammatory process involving adipose tissues.  It has a diverse array of functions, of which the most important one is to regulate energy balance.  It does so by decreasing the appetite or energy intake and by increasing energy expenditure. Hence, it is rightly called as the satiety hormone.  By regulating energy metabolism, it also regulates the body fat stores. Hence, the leptin levels in the body correspond to the adipose tissue mass.  More recently, leptin was found to mediate the secretion and peripheral tissue sensitivity of the hormone insulin.  The role of leptin in altering glucose metabolism and insulin sensitivity in T2DM are not fully explored. Since IR is the basis behind the development of T2DM, we designed the present study to estimate the levels of leptin and IR (using HOMA-IR) in newly diagnosed T2DM patients, in a view to determine the role of leptin in the development of IR in T2DM. The study is a descriptive study, done between March 2013 and March 2015. The study was approv Continue reading >>
Leptin Is Reduced In Lean Subjects With Type 2 Diabetes In Bangladesh
Leptin Is Reduced in Lean Subjects With Type 2 Diabetes in Bangladesh Mohammed Abu Sayeed, MBBS, DCM, MD, PHD1, Hajera Mahtab, MBCHB, DTM&H, FCPS, FRCP1, 1Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka, Bangladesh 2Department of Medicine, Lund University, Lund, Sweden Diabetes Care 2003 Feb; 26(2): 547-547. Although leptin levels are increased in obesity ( 1 ), obese subjects with type 2 diabetes display reduced leptin ( 2 4 ), which may be due to altered fat distribution ( 5 ). This study examined whether leptin levels are also reduced in lean subjects with type 2 diabetes. Fifty nonobese Bangladeshi women with type 2 diabetes (aged 37.2 1.3 years) were selected randomly from the Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) outpatient department (28 were on diet and exercise, and 22 were on oral hypoglycemic agents; HbA1c 9.6 0.8%). A total of 50 nondiabetic age- and BMI-matched health professional women (aged 33.4 1.9 years) served as control subjects. Circulating leptin (RIA; Linco, St. Charles, MO), BMI, waist-to-hip ratio (WHR), and mid-arm circumference (MAC) were measured. A 75-g oral glucose tolerance test (OGTT) was undertaken with measurements of glucose and insulin (radioimmunoassay). Diabetic subjects had lower leptin (11.1 1.6 vs. 16.2 1.9 ng/ml, P < 0.001), higher WHR (0.86 0.02 vs. 0.84 0.01; P = 0.034), and lower MAC (23.7 0.4 vs. 25.4 0.7 cm; P < 0.001) than nondiabetic subjects, without any difference in BMI (22.8 0.4 vs. 23.0 0.6 kg/m2). Leptin correlated to MAC (r = 0.46, P < 0.001) but not to WHR (r = 0.01). Although fasting insulin did not differ between the groups (84.2 16.6 vs. 92.7 34.2 pmol/l), the 60-min insulin l Continue reading >>
Insulin-resistant Patients With Type 2 Diabetes Mellitus Have Higher Serum Leptinlevels Independently Of Body Fat Mass.
Insulin-resistant patients with type 2 diabetes mellitus have higher serum leptinlevels independently of body fat mass. Fischer S(1), Hanefeld M, Haffner SM, Fusch C, Schwanebeck U, Khler C, Fcker K,Julius U. (1)Institute of Clinical Metabolic Research, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Fetscherstrasse 74, 01307 Dresden, Germany. In obese people, an increase of plasma leptin levels is well-known and is seen asa consequence of the increased body fat mass. Moreover, a relationship betweenfasting concentrations of leptin and insulin has been described. Hyperinsulinemiais considered to be indicative of insulin resistance. We aimed at elucidating theinterrelations between leptin, insulin and insulin resistance in type 2 diabetic patients. Under metabolic ward conditions, we investigated 21 moderatelyoverweight men with type 2 diabetes. The patients had a mean age of 49.1 years, amean body mass index (BMI) of 26.8 kg/m(2), and a mean diabetes duration of 82.5 months. All patients were treated with diet alone. We measured fasting leptin andinsulin levels, body composition by determination of total body water, andinsulin resistance by euglycemic hyperinsulinemic clamp technique. At univariate analysis, fasting leptin level significantly and positively correlated with BMI(r=0.49, p=0.02) and with fasting insulin (r=0.69, p=0.001), while it negatively correlated with the glucose disposal rate (r=-0.62, p=0.002). Furthermore, leptinwas inversely correlated with HDL-cholesterol (r=-0.45, p=0.04). When excludingthe influence of body fat mass or of BMI in partial correlation analysis, thecorrelations between leptin and insulin or insulin sensitivity remainedsignificant. The relationship between insulin resistance (as measured directly inthe clamp e Continue reading >>
Association Between Plasma Leptin Levels And Diabetes Mellitus
Association Between Plasma Leptin Levels and Diabetes Mellitus Pavani Bandaru , M.D., M.P.H. and Anoop Shankar , M.D., Ph.D. Department of Community Medicine, West Virginia University School of Medicine, Morgantown, West Virginia. Address correspondence to: Anoop Shankar, M.D., Ph.D., Department of Medicine, West Virginia University School of Medicine, P.O. Box 9190, Morgantown, WV 26506-9190. E-mail: [email protected] This article has been cited by other articles in PMC. Leptin is an important adipose tissuederived hormone that has been shown to be involved in pathophysiological mechanisms related to cardiovascular disease and diabetes. However, few studies have examined the association between plasma leptin and diabetes mellitus in humans. Also, it is not clear if this association is present among women as well as in men. Therefore, we examined the association between plasma leptin levels and diabetes mellitus in a representative multiethnic sample of U.S. adults. We examined the 19881994 third National Health and Nutrition Examination Survey (NHANES III) participants >20 years of age (n = 5,599, 54.7% women). Plasma leptin levels were categorized into quartiles (women, <7.68 fg/L, 7.6813.18 fg/L, 13.1921.70 fg/L, >21.70 fg/L; men, <2.64 fg/L, 2.644.36 fg/L, 4.377.12 fg/L, >7.12 fg/L). Diabetes mellitus was defined as fasting glucose 126 mg/dL, non-fasting glucose 200 mg/dL or use of oral hypoglycemic medication or insulin (n = 395). Higher plasma leptin levels were initially found to be associated with diabetes mellitus after adjusting for age, sex, race/ethnicity, education, smoking, alcohol intake, hypertension, serum cholesterol and C-reactive protein [odds ratio (OR), 3.79; confidence interval (CI), 2.057.00; P trend <0.0001). However, when we additionally ad Continue reading >>
Plasma Leptin Concentrations Are Greater In Type Ii Diabetic Patients And Stimulate Monocyte Chemotactic Peptide-1 Synthesis Via The Mitogen-activated Protein Kinase/extracellular Signal-regulated Kinase Pathway - Sciencedirect
Volume 31, Issue 3 , September 2012, Pages 177-185 Plasma leptin concentrations are greater in type II diabetic patients and stimulate monocyte chemotactic peptide-1 synthesis via the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway Open Access funded by The Korean Society of Nephrology Leptin is an adipokine that is recently reported to be a biomarker of systemic inflammation. Although atherosclerosis causes cardiovascular diseases, it is not clear whether leptin contributes to the acceleration of this process. In this study, we investigated whether alterations of plasma leptin levels were related to diabetic nephropathy and systemic inflammation. In addition, we examined the physiologic action of leptin in cultured vascular smooth muscle cells (VSMCs). A total of 126 type 2 diabetic participants and 37 healthy controls were studied. The diabetic participants were divided into three groups according to stage of nephropathy. We investigated whether leptin induced monocyte chemotactic peptide-1 (MCP-1) synthesis through the mitogen-activated protein kinase (MAPK) pathway using cultured VSMCs. Plasma leptin concentrations were significantly higher in the diabetic group than in the controls. Plasma leptin levels were positively correlated with body mass index, fasting and postprandial blood glucose, hemoglobin A1c, total cholesterol, urinary albumin excretion, high-sensitivity C-reactive protein (hsCRP), and MCP-1 plasma levels, and negatively correlated with creatinine clearance values. In cultured VSMCs, leptin increased MCP-1 production in a dose-dependent manner, and this stimulating effect of leptin on MCP-1 expression was reversed by the MAPK (MEK) inhibitor PD98059. In addition, leptin stimulated the phosphorylation of MEK, extracellul Continue reading >>