A New Method For Targeted And Sustained Induction Of Type 2 Diabetes In Rodents
A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents Scientific Reportsvolume7, Articlenumber:14158 (2017) Type 2 diabetes is a chronic metabolic disorder that is becoming a leading cause of morbidity and mortality. The prolonged time-course of human type 2 diabetes makes modelling of the disease difficult and additional animal models and methodologies are needed. The goal of this study was to develop and characterise a new method that allows controlled, targeted and sustained induction of discrete stages of type 2 diabetes in rodents. Using adult, male rats, we employed a three-week high fat-diet regimen and confirmed development of obesity-associated glucose intolerance, a key feature of human type 2 diabetes. Next, we utilised osmotic mini-pumps to infuse streptozotocin (STZ; doses ranging 80200 mg/kg) over the course of 14-days to decrease insulin-producing capacity thus promoting hyperglycemia. Using this new approach, we demonstrate a dose-dependent effect of STZ on circulating glucose and insulin levels as well as glucose tolerance, while retaining a state of obesity. Importantly, we found that insulin secretion in response to a glucose load was present, but reduced in a dose-dependent manner by increasing STZ. In conclusion, we demonstrate a novel method that enables induction of discrete stages of type 2 diabetes in rodents that closely mirrors the different stages of type 2 diabetes in humans. Among the different forms of diabetes, type 2 diabetes accounts for approximately 90% of cases and current estimates indicate that by 2040, approximately 642 million world-wide people will be living with type 2 diabetes 1 , 2 . This is likely to be a conservative estimate given that for every case of diagnosed type 2 diabetes, we know there is Continue reading >>
Antidiabetic Effects Of Embelia Ribes Extract In High Fat Diet And Low Dose Streptozotocin-induced Type 2 Diabetic Rats
Antidiabetic effects of Embelia ribes extract in high fat diet and low dose streptozotocin-induced type 2 diabetic rats Antidiabetic effects of Embelia ribes extract in high fat diet and low dose streptozotocin-induced type 2 diabetic rats Embelia ribes is extensively used in the traditional Indian system of medicine for treatment of various disorders. We herein investigated the antidiabetic effects of E. ribes ethanolic extract in high fat diet (HFD) and low dose streptozotocin (STZ)-induced type 2 diabetes in Wistar rats. HFD-fed and low dose STZ (35mgkg1, i.p)-induced diabetic rats were treated with E. ribes extract (100 and 200mgkg1 day1) or metformin (180mgkg1 day1) for 21 days while continuing on HFD. Antihyperglycemic effects of E. ribes extract were demonstrated by significant reduction (p<0.01) in fasting blood glucose, hepatic glucose-6-phosphatase activity and increased glycogen content, whereas insulin sensitizing effects were seen during oral glucose tolerance testing. In addition, it significantly (p<0.01) restored the elevated systolic and diastolic blood pressure, reduced hepatic fatty degeneration, oxidative changes and the reversal of circulating levels of serum lipids, lipase, insulin, leptin and adiponectin towards normal in diabetic rats. These findings demonstrated the antidiabetic potential of E. ribes extract in HFD-fed and low dose STZ-treated diabetic rats. This may be associated with strong antioxidant potential and restoration of the serum levels of leptin, adiponectin, insulin and lipids. Keywords: type 2 diabetes , adiponectin , leptin , lipase , oxidative stress , high fat diet The prevalence of type 2 diabetes mellitus is rapidly rising around the world. India is the diabetes capital of the world with 62.4 million Indians having type 2 d Continue reading >>
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Effect Of Eugenia Jambolana On Streptozotocin-nicotinamideinduced Type-2 Diabetic Nephropathy In Rats
Effect of Eugenia Jambolana on Streptozotocin-Nicotinamideinduced type-2 Diabetic Nephropathy in Rats Godwin Selvaraj Esther1*, Alvin Jose Manonmani2 Research Scholar, PRIST University, Vallam, Thanjavur- 613403, Tamil Nadu, India./ Department of Pharmacology, Noorul Islam College of Dental Science, NIMS Medicity, Aralummoodu-695123, Trivandrum, Kerala, India. Department of Pharmacology, Swamy Vivekanandha College of Pharmacy, Elayampalayam, Tiruchengode-637205, Tamil Nadu, India. Corresponding Author: Godwin Selvaraj Esther Department of Pharmacology, Noorul Islam College of Dental Science, NIMS Medicity, Aralummoodu-695123, Trivandrum, Kerala, India. E-mail: [email protected] Date of Submission: 08-12-2013 Date of Acceptance: 17-01-2014 Conflict of Interest: NIL Source of Support: NONE Copyright: 2014 Godwin Selvaraj Esther et al, publisher and licensee IYPF. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. Related article at Pubmed , Scholar Google Visit for more related articles at International Journal of Drug Development and Research The chronic type-2 diabetes mellitus leads to diabetic nephropathy, which is one of the major microvascular complication of end stage renal disease worldwide and causes premature death in diabetic patients. The objective of the present investigation was to evaluate the antidiabetic activity and protective effect of diabetic induced nephropathy of ethanolic extract of seeds of Eugenia jambolana (SEEJ) by using in-vitro and in-vivo models. The in-vitro antidiabetic effect was studied by glucose uptake assay in lymphocyte culture preparation. The in-vivo antidiabetic activity and the effect on diabetic nephropathy was evaluated using streptozotocin-nicotinam Continue reading >>
Streptozotocininduced Diabetic Models In Mice And Rats
Please review our Terms and Conditions of Use and check box below to share full-text version of article. I have read and accept the Wiley Online Library Terms and Conditions of Use. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Streptozotocin (STZ) is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZinduced insulin deficiency and hyperglycemia in mice and rats. Also described are protocols for creating animal models for type 2 diabetes using STZ. These animals are employed for assessing the pathological consequences of diabetes and for screening potential therapies for the treatment of this condition. 2015 by John Wiley & Sons, Inc. Yu-Ting Tseng, Wan-Hsuan Chang, Chih-Cheng Lin, Fang-Rong Chang, Pao-Chu Wu and Yi-Ching Lo, Protective effects of Liuwei dihuang water extracts on diabetic muscle atrophy, Phytomedicine, 10.1016/j.phymed.2018.09.032, 53, (96-106), (2019). Liming Yu, Zhi Li, Xue Dong, Xiaodong Xue, Yu Liu, Shu Xu, Jian Zhang, Jinsong Han, Yang Yang and Huishan Wang, Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling, Oxidative Medicine and Cellular Longevity, 2018, (1), (2018). Haiyan Wei, Qiaoyun Hu, Junxia Wu, Chenjuan Yao, Lingfei Xu, Fengjun Xing, Xinyuan Zhao, Shali Yu, Xiaoke Wang and Gang Chen, Molecular mechanism of the increased tissue uptake of trivalent inorganic arsenic in mice with type 1 diabetes mellitus, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2018.06.029, 504, 2, (393-399), (2018). Jay Parikh, Alice Zemljic-Harpf, Johnny F Continue reading >>
![[full Text] Streptozotocin-induced Type 1 Diabetes In Rodents As A Model For Study | Dmso](https://diabetestalk.net/images/NYlAIOcyUohZJavR.jpg)
[full Text] Streptozotocin-induced Type 1 Diabetes In Rodents As A Model For Study | Dmso
Editor who approved publication: Professor Ming-Hui Zou Department of Pharmaceutical Sciences, UNT System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX, USA Abstract: Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic mechanisms of diabetes and its complications. Streptozotocin (STZ)-induced diabetic animal models are useful platforms for the understanding of cell glucotoxicity in diabetes. As diabetes induced by a single STZ injection is often referred to as type 1 diabetes that is caused by STZs partial destruction of pancreas, one question often being asked is whether the STZ type 1 diabetes animal model is a good model for studying the mitochondrial mechanisms of cell glucotoxicity. In this mini review, we provide evidence garnered from the literature that the STZ type 1 diabetes is indeed a suitable model for studying mitochondrial mechanisms of diabetic cell glucotoxicity. Evidence presented includes: 1) continued cell derangement is due to chronic hyperglycemia after STZ is completely eliminated out of the body; 2) STZ diabetes can be reversed by insulin treatment, which indicates that cell responds to treatment and shows ability to regenerate; and 3) STZ diabetes can be ameliorated or alleviated by administration of phytochemicals. In addition, mechanisms of STZ action and fundamental gaps in understanding mitochondrial mechanisms of cell dysfunction are also discussed. Keywords: diabetes, cell, glucotoxicity, mitochondria, redox imbalance, streptozotocin Diabetes mellitus and its complications are chronic glucotoxicity diseases. The concept of cell glucotoxicity (and other cells as well) implicates that persistent excessive glucose can exert adverse or toxic effect on cell function after the Continue reading >>
High-fat Diet/low-dose Streptozotocin-induced Type 2 Diabetes In Rats Impacts Osteogenesis And Wnt Signaling In Bone Marrow Stromal Cells
High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes in Rats Impacts Osteogenesis and Wnt Signaling in Bone Marrow Stromal Cells Contributed equally to this work with: Chao Qian, Chenyuan Zhu Affiliation: Department of Prosthodontics, School of Stomatology, Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, 200011, Peoples Republic of China Contributed equally to this work with: Chao Qian, Chenyuan Zhu Affiliation: Department of Prosthodontics, School of Stomatology, Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, 200011, Peoples Republic of China Affiliation: Department of Prosthodontics, School of Stomatology, Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, 200011, Peoples Republic of China * E-mail: [email protected] (FZ); [email protected] (XJ) Affiliation: Department of Prosthodontics, School of Stomatology, Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, 200011, Peoples Republic of China These authors also contributed equally to this work. * E-mail: [email protected] (FZ); [email protected] (XJ) Affiliation: Department of Prosthodontics, School of Stomatology, Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai, 200011, Peoples Republic of China These authors also contributed equally to this work. Bone regeneration disorders are a significant problem in patients with type 2 diabetes mellitus. Bone marrow stromal cells (BMSCs) are recognized as ideal seed cells for tissue engineerin Continue reading >>
How Can You Induce Type Ii Diabetes In Wistar Albino Rats Using Streptozotocin? How Much Concentration Should Be Prepared?
You can not induce Type-2 diabetics using Streptozotocin alone in adult rats. Please give High Fat Diet (Lard Conc 40-50%) for around 14 days. HFD should increase the body weight. On day 15 inject STZ, 45mg/kg in Citrate Buffer, i.p. route. At the end of 3rd week you can check fasting blood glucose levels. You have to standardise this method with some changes according to your facility. Try to standardise it on male rats, body weight range 140 to 160g (wistar or SD). OR If you want to use only STZ then try in rat pubs (age within 24h of birth). Single shot of STZ (90-100mg/kg, i.p. route) will work. Allow the pubs with mother up to weaning period. Then check the fasting blood glucose level. Apart from the answers given by Drs Hamza and Misra, we recently induced an animal model of type 2 diabetes using a new approache which has been published in the Pharmacological Reports on 2012 authored by Wilson RD and Islam MS. You can have a look on that paper which is accessible freely on the web or journal home page. Please let me know if you cannot get it from the web or journal any way. We have done streptozotocin induced diabetes in our research. Many rats die from very high glucose, osmotic diuresis, and volume depletion are the underlying factors for the high mortality of streptozotocin induced diabetes. This is not a sustainable model to investigate long-term effects of diabetes on organ system. Her is our publication citation: Clin Exp Hypertens Theory and Practice 1990; A12: 1021-1035. East Suffolk and North Essex Foundation Trust STZ can induce mild hyperglycaemia but in no way replicates the complex pathophysiology of T2DM. So I agree with Dr Misra, in some respects. However, the best models are the various rat strains available specifically as models of T2DM. STZ ind Continue reading >>
Blood Glucose Level And Lipid Profile Of Streptozotocin-induced Diabetes Rats Treated With Sodium Alginate From Sargassum Crassifolium
Blood Glucose Level and Lipid Profile of Streptozotocin-induced Diabetes Rats Treated with Sodium Alginate from Sargassum crassifolium The objective of this study was to evaluate the potential effect of sodium alginate from Sargassum crassifolium on glucose level and lipid profile in streptozotocin-induced diabetes rats. Sodium alginate extract of S. crassifolium 200, 400 and 600 mg kg1 was administered orally to streptozotocin-induced diabetes rats, once daily for 15 days. The result obtained were then compared with normal control (non-diabetic+normal saline 0.9% p.o), negative control (diabetes+CMC-Na 0.5% p.o) and positive control (diabetic+glibenclamide 5 mg kg1 p.o). The glucose level, lipid profile and body weight were measured on normal condition (baseline) on 0, 5th, 10th, 15th day and pancreatic histopathological study were done on 15th day. Sodium alginate extract of S. crassifolium had yield 23.82% db, viscosity of 521 cps and a water content of 16.18% db. Sodium alginate 600 mg kg1 significantly reduce level preprandial glucose, postprandial glucose and total cholesterol compared negative control and did not have significant difference with positive control. Levels of triglycerides and LDL-c throughout the treatment groups had significant differences with the negative control. Meanwhile, the levels of HDL-c throughout the treatment group did not have significant differences. Necrosis was found in all streptozotocin-induced rats. The higher alginate doses given, getting better lower the levels of glucose and total cholesterol. Received: December 21, 2015; Accepted: February 01, 2016; Published: March 15, 2016 The term diabetes mellitus describes a metabolic disorder of multiple aetiology characterized by cronic hyperglycaemia with disturbances of carbohydrat Continue reading >>
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Animal Models In Diabetes And Pregnancy
Laboratory of Reproduction and Metabolism, Centro de Estudios Farmacologicos y BotanicosConsejo Nacional de Investigaciones Cientificas y Tecnicas, School of Medicine, University of Buenos Aires, 1121ABG Buenos Aires, Argentina Address all correspondence and requests for reprints to: Alicia Jawerbaum, Laboratory of Reproduction and Metabolism, Centro de Estudios Farmacologicos y BotanicosConsejo Nacional de Investigaciones Cientificas y Tecnicas-School of Medicine, University of Buenos Aires. Paraguay 2155, 17th floor (C1121ABG), 1121ABG Buenos Aires, Argentina. Search for other works by this author on: Laboratory of Reproduction and Metabolism, Centro de Estudios Farmacologicos y BotanicosConsejo Nacional de Investigaciones Cientificas y Tecnicas, School of Medicine, University of Buenos Aires, 1121ABG Buenos Aires, Argentina Search for other works by this author on: Endocrine Reviews, Volume 31, Issue 5, 1 October 2010, Pages 680701, Alicia Jawerbaum, Veronica White; Animal Models in Diabetes and Pregnancy, Endocrine Reviews, Volume 31, Issue 5, 1 October 2010, Pages 680701, The worldwide increase in the incidence of diabetes, the increase in type 2 diabetes in women at reproductive ages, and the cross-generation of the intrauterine programming of type 2 diabetes are the bases for the growing interest in the use of experimental diabetic models in order to gain insight into the mechanisms of induction of developmental alterations in maternal diabetes. In this scenario, experimental models that present the most common features of diabetes in pregnancy are highly required. Several important aspects of human diabetic pregnancies such as the increased rates of spontaneous abortions, malformations, fetoplacental impairments, and offspring diseases in later life can be appr Continue reading >>
How Can We Induce Type-1 Diabetes And Type-2 Diabetes In Rats?
sir i'm working on mice. multiple low doses of STZ produced diabetes. i tried according to article sited on global journal of pharmacology. please go trough it may be of some use to u.Global Journal of Pharmacology, 3 (2): 81-84, 2009. National Institute Of Business Management sri satya sai medical college and research institute. Chennai. sir u can induce type 2 diabetes by STZ and it is the most common method used to screen the anti diabetic drugs..We can do it with alloxan also but its irreversible and 30% of the animals will die.. Sri Chandrasekharendra Saraswathi Viswa Mahavidyalaya University Few are telling that 30% rats will die with 50 mg/kg, in my experience not so. I am lucky t o get less than that death rate. Once even i gave up to 70mg/kg For type 1 diabetes: 60 mg/kg STZ by i.p. route most commonly used. For Type 2 diabetes: 65 mg/kg STZ by i.p., after the Nicotinamide 110 mg/kg, i.p. administration. Neither one of those models is a model for type 2 diabetes. Use of STZ is only an approximate model of type 1, nothing more. Your paper will be trashed by reviewers if you say that you have a model of type 2 diabetes when using STZ alone. type 1 diabetes is induced by alloxan. High dose of alloxan induced type 1 diabetes and low dose of alloxan induce type 2 diabetes in previous reports. u can either go for fructose fed diabetic model. high callories fed diabetic model will be type II type of.(can use Insulin receptor inhibitors or insulin antagonists- i dont know wether are used or not). Neonatal STZ induced diabetes is a model approximating type II diabetes in rats. You must inject the drug i.p. (or i.v.) in the rat at day 1-4 after birth. It is better to choice male individuals, in which diabetes shows lesser number of variables. The rats show a rise of gly Continue reading >>
The Use Of Animal Models In The Study Of Diabetes Mellitus
Abstract Animal models have enormously contributed to the study of diabetes mellitus, a metabolic disease with abnormal glucose homeostasis, due to some defect in the secretion or the action of insulin. They give researchers the opportunity to control in vivo the genetic and environmental factors that may influence the development of the disease and establishment of its complications, and thus gain new information about its handling and treatment in humans. Most experiments are carried out on rodents, even though other species with human-like biological characteristics are also used. Animal models develop diabetes either spontaneously or by using chemical, surgical, genetic or other techniques, and depict many clinical features or related phenotypes of the disease. In this review, an overview of the most commonly used animal models of diabetes are provided, highlighting the advantages and limitations of each model, and discussing their usefulness and contribution in the field of diabetes research. Type I Diabetes (T1DM) Models T1DM, a multifactorial autoimmune disease involving genetic and environmental factors, is hallmarked by T-cell and macrophages-mediated destruction of pancreatic β-cells, resulting in irreversible insulin deficiency. Diabetic ketoacidosis, a T1DM immediate consequence, can be fatal without treatment, while the long-term vascular T1DM complications affecting several organs and tissues can significantly affect life expectancy. There is no doubt that T1DM susceptibility is MHC-dependent and MHC genes account for approximately 50% of the total contribution to the disease. However, although to date studies corroborate that both HLA-DR and HLA-DQ genes are important in determining disease risk, the effects of individual alleles may be modified by the h Continue reading >>
What Is The Best Method To Induce Type 2 Diabetes Rat Model??
From my experiments and observations, the combined administration of streptozotocin (STZ) with and nicotinamide (NA) to adult rats results in the development of a diabetic syndrome, characterizes by moderate and stable hyperglycemia. This syndrome is similar to T2DM in humans and is thus a suitable model for short and long-term studies. In our laboratory, for induction of non-insulin dependent diabetes mellitus (NIDDM), the method of Masiello et al [Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, Novelli M, Ribes G: Experimental NIDDM: development of a new model in adult rats administered STZ and NA. Diabetes 1998; 47, 224-9] was used. In the majority of experiments, NA is given to rats 15 min before STZ to induce NIDDM in rats. Administration of the diabetogenic dose of STZ to rats causes a decrease in body weight; however, this is attenuated by pre-treatment of animals with NA. Numerous studies have also demonstrated that the STZ-induced increase in blood glucose is significantly blunted when NA is administered prior to STZ. The advantageous effect of NA on blood glucose is due to the protection of B-cells against demage of STZ and is accompanied by increased blood insulin. The severity of diabetes in experimental rats strongly depends on the doses of STZ and NA given to these animals (Check out the following minireview). Continue reading >>
A Practical Guide For Induction Of Type-2 Diabetes In Rat: Incorporating A High-fat Diet And Streptozotocin - Sciencedirect
A practical guide for induction of type-2 diabetes in rat: Incorporating a high-fat diet and streptozotocin Author links open overlay panel SevdaGheibiab KhosrowKashfic AsgharGhasemia Feeding a high fat diet (HFD) to rats causes insulin resistance. Administration of low-dose streptozotocin (STZ) causes -cell dysfunction. Combining a HFD with low-dose STZ induces type-2 diabetes (T2D). HFD-STZ-T2D exhibits the metabolic characteristics similar to those seen in human T2D. Prevalence of diabetes, a serious public health problem is rapidly increasing worldwide. Type-2 diabetes is the common form of diabetes characterized by insulin resistance and abnormalities in insulin production. Despite the current development of therapeutic agents, there is no effective treatment without side effects; it is therefore necessary to find new prevention strategies and better treatments. For this purpose animal models of diabetes are appropriate tools, of which rodents due to the short generation time and economic considerations are the first choice. The aim of this review is to present features of a frequently used model of type-2 diabetes in rat, induced by a high fat diet and streptozotocin, taking into account its advantages/disadvantages and presenting a practical guide. Continue reading >>
Histological And Biochemical Evaluation Of The Antidiabetic Potentials Of S-allyl-cysteine And Mangiferin In Type 2 Diabetic Rat Models Iliya I A, Mohammed B, Akuyam S A, Yaro J D, Timbuak J A, Tanko M, Nok A J - Sub-saharan Afr J Med
Biochemical and histological assessments was carried out on the anti-diabetic potentials of s-allyl-cysteine (SAC) and mangiferin (MAN) in Wistar rats models induced with type 2 diabetes mellitus by feeding the rats with a high fat diet for 10 weeks followed by a low dose injection of streptozotocin (40 mg/kg). Therapeutic interventions with 50 mg/kg body weight of (SAC) and 40 mg/kg body weight of (MAN) and a combined therapy (COM) of both SAC and MAN in equal volume ratios (1:1) for 14 days showed that there was a significant improvement in the glucose tolerance ability of the diabetic treated rats (P < 0.05). Consequently, the activities of hepatic pathophysiological enzymes (Alanine aminotransferase, ALT; Aspartate aminotransferase, AST; Alkaline phosphatase, ALP), as well as glycosylated haemoglobin levels were significantly ameliorated in the diabetic treated rat models (P < 0.05). Histomorphometrical examinations of stained pancreatic tissue sections showed a reduction in the total surface area of islets in the diabetic control rats which was however significantly improved in the diabetic treated rats (P < 0.05) except the COM treated group. Subsequent histomorphological evaluation also showed necrosis and vacuolization of islet -cells to be reasonably reduced in the diabetic treated rats but to a lesser extent in the COM treated group. Keywords:Glycosylated haemoglobin, histomorphological, histomorphometrical, insulin, liver enzymes, mangiferin, s-allyl-cysteine, type 2 diabetes mellitus Iliya I A, Mohammed B, Akuyam S A, Yaro J D, Timbuak J A, Tanko M, Nok A J. Histological and biochemical evaluation of the antidiabetic potentials of s-allyl-cysteine and mangiferin in type 2 diabetic rat models. Sub-Saharan Afr J Med 2016;3:32-40 Iliya I A, Mohammed B, Akuyam Continue reading >>
Effect Of Atorvastatin On Pharmacology Of Sitagliptin In Streptozotocin-nicotinamide Induced Type-ii Diabetes In Rats
Received date: December 17, 2014; Accepted date: December 31, 2014; Published date: January 07, 2015 Citation: Eggadi V, Sheshagiri SBB, Devandla A, Dasi N, Kulundaivelu U, et al. (2015) Effect of Atorvastatin on Pharmacology of Sitagliptin in Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats. Biol Med 7:225. doi:10.4172/0974-8369.1000225 Copyright: 2015 Eggadi V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Prolonged type 2 diabetes mellitus (Type2DM) may lead to high risk of cardiovascular disease (CVD) requiring a global therapeutic approach. Statin therapy has proven its efficacy in reducing CVD events in Type2 DM patients. Dipeptidyl peptidase-4 inhibitors (gliptins), which are increasingly used to target hyperglycemia. In the present study type 2 DM in rats by i.p. Administration of Streptozotocin (STZ); (60 mg/kg) -Nicotinamide (120 mg/kg). Diabetes induced rats were divided into groups and treated with Sitagliptin alone and in combination with atorvastatin for 7 days. Blood samples were collected by retro orbital puncture. Mean glucose concentration was measured by GODPOD method using commercial glucose kits and sitagliptin in plasma was estimated by RP-HPLC method using methanol: water (60:40 v/v, containing 10 mM Tris and 10 mM Triethylamine) was adjusted to pH 9.0 using 1 mol/L hydrochloric acid. The blood glucose lowering activity of sitagliptin was increased by the presence of atorvastatin in diabetic rats. The pharmacokinetic (PK) and pharmacodynamic (PD) parameters of sitagliptin in diabetic rats were significantly changed in the presence of atorvas Continue reading >>
