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Alloxan Induced Diabetes Mechanisms And Effects

The Mechanisms Of Alloxan- And Streptozotocin-induced Diabetes

The Mechanisms Of Alloxan- And Streptozotocin-induced Diabetes

, Volume 51, Issue2 , pp 216226 | Cite as The mechanisms of alloxan- and streptozotocin-induced diabetes Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the GLUT2 glucose transporter. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species (ROS) in a cyclic redox reaction with its reduction product, dialuric acid. Autoxidation of dialuric acid generates superoxide radicals, hydrogen peroxide and, in a final iron-catalysed reaction step, hydroxyl radicals. These hydroxyl radicals are ultimately responsible for the death of the beta cells, which have a particularly low antioxidative defence capacity, and the ensuing state of insulin-dependent alloxan diabetes. As a thiol reagent, alloxan also selectively inhibits glucose-induced insulin secretion through its ability to inhibit the beta cell glucose sensor glucokinase. Following its uptake into the beta cells, streptozotocin is split into its glucose and methylnitrosourea moiety. Owing to its alkylating properties, the latter modifies biological macromolecules, fragments DNA and destroys the beta cells, causing a state of insulin-dependent diabetes. The targeting of mitochondrial DNA, thereby impairing the signalling function of beta cell mitochondrial metabolism, also explains how streptozotocin is able to inhibit glucose-induced insulin secretion. AlkylationAlloxan diabetesCytotoxic glucose analoguesPancreatic beta cell toxicityReactive oxygen speciesStreptozotocin diabetes Alloxan and streptozotocin are the most prominent diabetogenic chemicals in diabetes research. Both are cytotoxic glucose analogues. Although their cytotoxicity is achieved via different pathways, their mechanisms of beta cell selecti Continue reading >>

Anti-diabetic Activity Of Ipomoea Batatas Leaves Extract: Effects On Hepatic Enzymes In Alloxan-induced Diabetic Rats

Anti-diabetic Activity Of Ipomoea Batatas Leaves Extract: Effects On Hepatic Enzymes In Alloxan-induced Diabetic Rats

Anti-Diabetic Activity of Ipomoea batatas Leaves Extract: Effects on Hepatic Enzymes in Alloxan-Induced Diabetic Rats Diabetes mellitus is the most common endocrine disorder of man, whose devastating effect is increasing by the day and severity almost at epidemic level. This study was carried out to investigate the anti-diabetic activity of Ipomoea batatas (sweet potato) leaves extract and its effect on hepatic enzymes, total protein and albumin in alloxan induced diabetic rats . A total of twenty animals was divided into four experimental groups consisting of five animals each. The groups included a positive control, negative control, diabetic-treated Ipomoea batatas and Diabetic-treated tolbutamide for 14 days. All were fed normal diet ad libitum. After the treatment a significant reduction was observed in fasting serum glucose levels in the treated diabetics rats. There was a significant (p<0.05) reduction of feed and water intakes by the animals after the treatment with Ipomoea batatas and tolbutamide. Treatment also improved the weight gain compared to untreated diabetic rats . Alkaline phosphatase activity in the diabetes untreated is significantly higher compared to that normal and treated animal and others treated with extract and tolbutamide. A Similar trend was observed in the Aspartate transaminase and Alanine transaminase activity, respectively, the reversed was observed in the albumin and total protein level, respectively. Hence, the result shows that the extract is not toxic and possesses anti-diabetic properties. Received: June 22, 2015; Accepted: August 06, 2015; Published: August 13, 2015 Diabetes Mellitus (DM), a common endocrine disorder of man, is considered one of the major health concerns all over the world today ( Rohilla and Ali, 2012 ). It is a Continue reading >>

Studies On The Mechanism Of Alloxan Diabetes

Studies On The Mechanism Of Alloxan Diabetes

STUDIES ON THE MECHANISM OF ALLOXAN DIABETES The Department of Medicine, Medical School, University of Chicago CHICAGO, ILLINOIS Search for other works by this author on: The Department of Medicine, Medical School, University of Chicago CHICAGO, ILLINOIS Search for other works by this author on: Endocrinology, Volume 35, Issue 4, 1 October 1944, Pages 241248, MARTIN G. GOLDNER, GEORGE GOMORI; STUDIES ON THE MECHANISM OF ALLOXAN DIABETES, Endocrinology, Volume 35, Issue 4, 1 October 1944, Pages 241248, IN APRIL 1943 Shaw Dunn, Sheehan and McLetchie announced their discovery that alloxan, the ureide of mesoxalic acid, causes necrosis of the pancreatic islet cells. In the meantime, the diabetogenic action of alloxan has been demonstrated almost simultaneously by Dunn and his associates in Glasgow (1943), by Bailey and Bailey in Boston (1943), and by Goldner and Gomori in Chicago (1943). Alloxan diabetes has been produced in rats, rabbits, dogs, monkeys, and cats (Goldner and Gomori, 1944). Anatomically, this diabetes is characterized by the selective necrosis of the beta cells in the islets of Langerhans. Prior to the establishment of the diabetes, the blood sugar of alloxan treated animals shows a characteristic triphasic reaction, which was first observed by Jacobs in 1937. An immediate hyperglycemia which reaches its peak within two or three hours, is followed by a severe, often fatal hypoglycemia, which after a duration of several hours yields to the final hyperglycemia (Fig. 1). Copyright 1944 by The Endocrine Society Continue reading >>

The Mechanisms Of Plant Rhizome Curcuma Longa Action On Carbohydrate Metabolism In Alloxan - Induced Diabetes Mellitus Rats

The Mechanisms Of Plant Rhizome Curcuma Longa Action On Carbohydrate Metabolism In Alloxan - Induced Diabetes Mellitus Rats

The Mechanisms of PLANT Rhizome Curcuma Longa Action on Carbohydrate Metabolism in Alloxan - Induced Diabetes Mellitus Rats , Koroshchenko G.A. 1 , Gajdarova A.P. 1 , Lukanina S.N. 1 , Subotyalov M.A. 1 1Department of Anatomy, Physiology and Life Safety FSBEI HPE Novosibirsk State Pedagogical University, Novosibirsk In experiments on rats with the alloxan-induced diabetes model the mechanisms of the turmeric (Curcuma longa) rhizomes influence on the various links in carbohydrate metabolism were studied. For this purpose, the glucose concentration in whole blood, titer hormones of insulin, C-peptide and cortisol in plasma, content of glycogen in the liver, structural and functional organization of the islet apparatus of the rats pancreas were studied. Under the influence of the turmeric rhizomes intake in rats with diabetes, as compared with the same animals on a standard diet, there were less expressed increasing of the glucose concentration in blood, increasing of the insulin and C-peptide concentration in plasma, lowering the concentration of cortisol and reliable increasing of the glycogen level in the liver. In the study of the microstructure of samples of pancreatic tissue of experimental animals on the background of the turmeric intake, the most preservation of the islet apparatus in comparison with a group of animals in conditions of a standard diet was marked. The results indicate the positive impact of the Curcuma longa rhizomes on the homeostatic mechanisms of the carbohydrate metabolism regulation in the alloxan-induced diabetes.Aim. The effects of the plant rhizome Curcuma longa as a food additive were studied on different processes of carbohydrate metabolism: glucose concentration in blood, concentration of hormones - insulin and C-peptide in plasma, conte Continue reading >>

Raw Camel Milk Properties On Alloxan-induced Diabetic Wistar Rats

Raw Camel Milk Properties On Alloxan-induced Diabetic Wistar Rats

Romanian Journal of Diabetes Nutrition and Metabolic Diseases The Journal of Romanian Society of Diabetes Nutrition and Metabolic Diseases Source Normalized Impact per Paper (SNIP) 2016: 0.133 *Prices in US$ apply to orders placed in the Americas only. Prices in GBP apply to orders placed in Great Britain only. Prices in represent the retail prices valid in Germany (unless otherwise indicated). Prices are subject to change without notice. Prices do not include postage and handling if applicable. RRP: Recommended Retail Price. Raw Camel Milk Properties on Alloxan-Induced Diabetic Wistar Rats Department of Biology, Faculty of Natural and Life Sciences, Hassiba Ben Bouali University Chlef, Algeria Department of Biology, Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi-bel-Abbes, Algeria Published Online: 2017-04-04 | DOI: Background and aims: Diabetes is one of the most frequent and serious chronic diseases in humans all over the world. The aim of our study was to evaluate the antidiabetic activity of camel milk on serum glucose and lipid profile of alloxan-induced diabetic rats. Materials and methods: Diabetes was induced in Wistar albino rats by intraperitoneal injection of alloxan (120 mg/kg BW once). Albino rats each weighing 180-230g were divided into 3 equal groups (n=10) as following: G1 - normal rats fed on normal diet, G2 - diabetic rats fed on normal diet, and G3 - diabetic rats were fed with raw camel milk. Fasting blood glucose was measured on days 0, 1, 7, 14, 21 and 30 while lipid profile was assessed at day 30. Results: After four weeks of feeding, data indicated a significant decrease (p<0.05) of mean blood glucose in G3 group (133.803.22 mg/dL) as compared with G2 diabetic rats (199.6 7.33 mg/dL). Data also revealed significant lower Continue reading >>

Altered Glucose Metabolism And Hypoxic Response In Alloxan-induced Diabetic Atherosclerosis In Rabbits

Altered Glucose Metabolism And Hypoxic Response In Alloxan-induced Diabetic Atherosclerosis In Rabbits

Altered glucose metabolism and hypoxic response in alloxan-induced diabetic atherosclerosis in rabbits Yunosuke Matsuura , Atsushi Yamashita , Yan Zhao , Takashi Iwakiri , Kazuaki Yamasaki , Chihiro Sugita , [...view 6 more...], Chihiro Koshimoto , Kazuo Kitamura , Keiichi Kawai , Nagara Tamaki , Songji Zhao , Yuji Kuge , Yujiro Asada [ view less ] Affiliations: Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan Affiliation: Department of Tracer Kinetics and Bioanalysis, Graduate School of Medicine, Hokkaido University, Sapporo, Japan Affiliations: Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan Affiliation: Department of Tracer Kinetics and Bioanalysis, Graduate School of Medicine, Hokkaido University, Sapporo, Japan Affiliations: Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan, Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Kyusyu University of Health and Welfare, Nobeoka, Japan Affiliation: Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan Affiliation: Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan Affiliations: Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan, Biomedical Imaging Research Center, University of Fukui, Fukui, Japan Affiliation: Department of Nuclear Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan Affiliation: Department of Tracer Kinetics and Bioanalysis Continue reading >>

Alloxan - Wikipedia

Alloxan - Wikipedia

Alloxan, sometimes referred to as alloxan hydrate, refers to the organic compound with the formula OC(N(H)CO)2C(OH)2. It is classified as a derivative of pyrimidine . The anhydrous derivative (OC(N(H)CO)2CO is also known as well as a dimeric derivative. These are some of the earliest known organic compounds. They also exhibit a variety of biological activities. The compound was discovered by Justus von Liebig and Friedrich Whler. It is one of the oldest named organic compounds. It was originally prepared in 1818 by Brugnatelli (1761-1818) [3] [4] and was named in 1838 by Whler and Liebig . [5] The name "Alloxan" emerged from an amalgamation of the words " allantoin " and "Oxalsure" ( oxalic acid ). The alloxan model of diabetes was first described in rabbits by Dunn, Sheehan and McLetchie in 1943. [6] The name is derived from allantoin , a product of uric acid excreted by the fetus into the allantois , and oxaluric acid derived from oxalic acid and urea , found in urine . Alloxan was used in the production of the purple dye murexide , discovered by Carl Wilhelm Scheele in 1776. Murexide is the product of the complex in-situ multistep reaction of alloxantin and gaseous ammonia . Murexide results from the condensation of the unisolated intermediate uramil with alloxan, liberated during the course of the reaction. Murexide dye (right) from reaction of alloxantin (left) Scheele sourced uric acid from human calculi (such as kidney stones ) and called the compound lithic acid. William Prout investigated the compound in 1818 and he used boa constrictor excrement with up to 90% ammonium acid urate. In the chapter "Nitrogen" of his memoir The Periodic Table , Primo Levi tells of his futile attempt to make alloxan for a cosmetics manufacturer who has read that it can cause perma Continue reading >>

Streptozotocin-induced Diabetes Models: Pathophysiological Mechanisms And Fetal Outcomes

Streptozotocin-induced Diabetes Models: Pathophysiological Mechanisms And Fetal Outcomes

Streptozotocin-Induced Diabetes Models: Pathophysiological Mechanisms and Fetal Outcomes D. C. Damasceno ,1,2 A. O. Netto ,1 I. L. Iessi ,1 F. Q. Gallego ,1 S. B. Corvino ,1 B. Dallaqua ,1 Y. K. Sinzato ,1 A. Bueno ,1 I. M. P. Calderon ,1and M. V. C. Rudge 1 1Laboratory of Experimental Research on Gynecology and Obstetrics, Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP-Universidade Estadual Paulista, Distrito de Rubio Jnior S/N, 18618-970 Botucatu, SP, Brazil 2Department of Gynecology and Obstetrics, Botucatu Medical School, UNESP-Univsidade Estadual Paulista, Distrito de Rubio Jnior S/N, 18618-970 Botucatu, SP, Brazil Received 14 March 2014; Revised 30 April 2014; Accepted 14 May 2014; Published 27 May 2014 Copyright 2014 D. C. Damasceno et al. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Hyperglycemia enhances the production of reactive oxygen species, leading to oxidative stress, which is involved in diabetic teratogenesis. It has also been suggested that maternal diabetes alters embryonic gene expression, which might cause malformations. Due to ethical issues involving human studies that sometimes have invasive aspects and the multiplicity of uncontrolled variables that can alter the uterine environment during clinical studies, it is neces Continue reading >>

Alloxan-induced Diabetes Causes Morphological And Ultrastructural Changes In Rat Liver That Resemble The Natural History Of Chronic Fatty Liver Disease In Humans

Alloxan-induced Diabetes Causes Morphological And Ultrastructural Changes In Rat Liver That Resemble The Natural History Of Chronic Fatty Liver Disease In Humans

Copyright © 2015 Amanda Natália Lucchesi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Purpose. This study evaluated the long-term effects of alloxan-induced diabetes in rat liver. Methods. Thirty nondiabetic control rats (NC) and 30 untreated diabetic (UD) rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Clinical and laboratory parameters were assessed. Fresh liver weight and its relationship with body weight were obtained, and liver tissue was analyzed. Results. UD rats showed sustained hyperglycemia, high glycosylated hemoglobin, and low plasma insulin. High serum levels of AST and ALT were observed in UD rats after 2 weeks, but only ALT remained elevated throughout the experiment. Fresh liver weight was equal between NC and UD rats, but the fresh liver weight/body weight ratio was significantly higher in UD rats after 14 and 26 weeks. UD rats showed liver morphological changes characterized by hepatic sinusoidal enlargement and micro- and macrovesicular hepatocyte fatty degeneration with progressive liver structure loss, steatohepatitis, and periportal fibrosis. Ultrastructural changes of hepatocytes, such as a decrease in the number of intracytoplasmic organelles and degeneration of mitochondria, rough endoplasmic reticulum, and nuclei, were also observed. Conclusion. Alloxan-induced diabetes triggered liver morphological and ultrastructural changes that closely resembled human disease, ranging from steatosis to steatohepatitis and liver fibrosis. 1. Introduction An association between type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease ( Continue reading >>

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An Error Occurred Setting Your User Cookie

An Error Occurred Setting Your User Cookie This site uses cookies to improve performance. If your browser does not accept cookies, you cannot view this site. There are many reasons why a cookie could not be set correctly. Below are the most common reasons: You have cookies disabled in your browser. You need to reset your browser to accept cookies or to ask you if you want to accept cookies. Your browser asks you whether you want to accept cookies and you declined. To accept cookies from this site, use the Back button and accept the cookie. Your browser does not support cookies. Try a different browser if you suspect this. The date on your computer is in the past. If your computer's clock shows a date before 1 Jan 1970, the browser will automatically forget the cookie. To fix this, set the correct time and date on your computer. You have installed an application that monitors or blocks cookies from being set. You must disable the application while logging in or check with your system administrator. This site uses cookies to improve performance by remembering that you are logged in when you go from page to page. To provide access without cookies would require the site to create a new session for every page you visit, which slows the system down to an unacceptable level. This site stores nothing other than an automatically generated session ID in the cookie; no other information is captured. In general, only the information that you provide, or the choices you make while visiting a web site, can be stored in a cookie. For example, the site cannot determine your email name unless you choose to type it. Allowing a website to create a cookie does not give that or any other site access to the rest of your computer, and only the site that created the cookie can read it. Continue reading >>

Pathomorphological Effects Of Alloxan Induced Acute Hypoglycaemia In Rabbits - Sciencedirect

Pathomorphological Effects Of Alloxan Induced Acute Hypoglycaemia In Rabbits - Sciencedirect

Volume 49, Issue 4 , December 2013, Pages 343-353 Pathomorphological effects of Alloxan induced acute hypoglycaemia in rabbits Author links open overlay panel MasoodSaleem Mira Open Access funded by Alexandria University Faculty of Medicine Alloxan is one of the frequently used beta-cytotoxic agents for the induction of Type-1 diabetes mellitus in animal models and is the drug of choice in rabbits. Its beta-cytotoxic action results in a sudden release of insulin leading to severe hypoglycaemia and even mortality if glucose therapy is not given. In the present investigation the pathological effects of alloxan induced acute hypoglycaemia were studied in rabbits. New Zealand White rabbits, 11.5 kg body weight, were administered alloxan @100 mg/kg b.w., as a single intravenous dose. Blood glucose levels were monitored (0 h, 20 min, 1 h, and then hourly up to 5 h) and clinical signs noted. Rabbits dead due to hypoglycaemia were necropsied and histopathology performed. Severe histopathological changes were observed especially in the brain (neuronal degeneration and necrosis), kidneys (nephrosis, nephritis) and liver (hepatosis, hepatitis) and also, other organs. Histopathological observation of beta-cytolysis was suggestive that the drug induced hypoglycaemia is insulin mediated. It was concluded that acute hypoglycaemia causes severe pathological changes and the alloxan induced immediate hypoglycaemia if not managed in time, might exacerbate the pathological effects of hyperglycaemia in the induced diabetic models. Continue reading >>

The Mechanisms Of Alloxan- And Streptozotocin-induced Diabetes.

The Mechanisms Of Alloxan- And Streptozotocin-induced Diabetes.

The mechanisms of alloxan- and streptozotocin-induced diabetes. Institute of Clinical Biochemistry, Hannover Medical School, 30623, Hannover, Germany. Diabetologia. 2008 Feb;51(2):216-26. Epub 2007 Dec 18. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the GLUT2 glucose transporter. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species (ROS) in a cyclic redox reaction with its reduction product, dialuric acid. Autoxidation of dialuric acid generates superoxide radicals, hydrogen peroxide and, in a final iron-catalysed reaction step, hydroxyl radicals. These hydroxyl radicals are ultimately responsible for the death of the beta cells, which have a particularly low antioxidative defence capacity, and the ensuing state of insulin-dependent 'alloxan diabetes'. As a thiol reagent, alloxan also selectively inhibits glucose-induced insulin secretion through its ability to inhibit the beta cell glucose sensor glucokinase. Following its uptake into the beta cells, streptozotocin is split into its glucose and methylnitrosourea moiety. Owing to its alkylating properties, the latter modifies biological macromolecules, fragments DNA and destroys the beta cells, causing a state of insulin-dependent diabetes. The targeting of mitochondrial DNA, thereby impairing the signalling function of beta cell mitochondrial metabolism, also explains how streptozotocin is able to inhibit glucose-induced insulin secretion. Continue reading >>

Anti-hypoglycemic And Hepatocyte-protective Effects Of Hyperoside From Zanthoxylum Bungeanum Leaves In Mice With High-carbohydrate/high-fat Diet And Alloxan-induced Diabetes

Anti-hypoglycemic And Hepatocyte-protective Effects Of Hyperoside From Zanthoxylum Bungeanum Leaves In Mice With High-carbohydrate/high-fat Diet And Alloxan-induced Diabetes

Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes Affiliations: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P.R. China, College of Forestry, Northwest A&F University, Xianyang, Shaanxi 712100, P.R. China Published online on: October 25, 2017 Copyright: Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License. Metrics: HTML 0 views | PDF 0 views Cited By (CrossRef): 0 citations The development of diabetes mellitus (DM) is accompanied by hyperglycemia-induced oxidative stress. Hyperoside is a major bioactive component in Zanthoxylumbungeanum leaves (HZL) and is a natural antioxidant. However, the effects of HZL on DM and its mechanisms of action remain undefined. The present study evaluated the anti-hypoglycemic and hepatocyte-protective effects of HZL in mice with diabetes induced by a high-carbohydrate/high-fat diet (HFD) and alloxan. We also aimed to eludicate the underlying mechanisms. Our resutls demonstrated that the administration of HZL significantly reduced body weight gain, serum glucose levels and insulin levels in diabetic mice compared with the vehicle-treated mice. In addition, the levels of dyslipidemia markers including total cholesterol, triglyceride and lowdensity lipoprotein cholesterol in the HFD-treated mice were markedly decreased. Further experiments using hepatocytes from mice revealed that HZL significantly attenuated liver injury associated with DM compared with vehicle treatment, as evidenced by lower levels of alanine aminotransferase and asparta Continue reading >>

Diabetes Mellitus Triggers Oxidative Stress In The Liver Of Alloxan-treated Rats: A Mechanism For Diabetic Chronic Liver Disease

Diabetes Mellitus Triggers Oxidative Stress In The Liver Of Alloxan-treated Rats: A Mechanism For Diabetic Chronic Liver Disease

5 – ORIGINAL ARTICLE MODELS, BIOLOGICAL Amanda Natália LucchesiI; Natália Tavares de FreitasII; Lucas Langoni CassettariIII; Sílvio Fernando Guideti MarquesIV; César Tadeu SpadellaV IFellow PhD degree, Postgraduate Program in General Basis of Surgery, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Technical procedures, acquisition of data, manuscript preparation IIGraduate student, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Technical procedures IIIFellow PhD degree, Postgraduate Program in General Basis of Surgery, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Acquisition, analysis and interpretation of data IVPhD, Assistant Professor, Methodist University of Piracicaba (UNIMEP), Sao Paulo, Brazil. Standardization of the method and biochemical dosages of the oxidative stress biomarkers VPhD, Full Professor, Department of Surgery and Orthopedics, Faculty of Medicine, UNESP, Botucatu-SP, Brazil. Supervised all phases of the study, manuscript writing, critical revision ABSTRACT PURPOSE: To investigate whether Diabetes mellitus chemically induced by alloxan is capable of changing, in the long term, the oxidative balance in the liver tissue of rats. METHODS: Sixty male Wistar rats, weighing 250-280g, were randomly distributed into two experimental groups: NG - 30 non-diabetic control rats; DG – 30 alloxan- induced diabetic rats without any treatment for the disease. Each group was further divided into three subgroups containing ten rats each, which were sacrificed after one, three and six months of follow-up, respectively. Blood glucose, urinary glucose, glycosylated hemoglobin and insulin were determined in the plasma of all animals at the beginning of the experiment and prior to all sacrifice periods. The concentrations of lipid hydroperoxides (HP) and t Continue reading >>

Hypoglycemic And Pancreatic Protective Effects Of Portulaca Oleracea Extract In Alloxan Induced Diabetic Rats

Hypoglycemic And Pancreatic Protective Effects Of Portulaca Oleracea Extract In Alloxan Induced Diabetic Rats

Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats 2Department of Biochemistry Faculty of Pharmacy, Misr International University, Km 28, Cairo-Ismailia road, Cairo PO Box 1, Heliopolis, Cairo Egypt 1Department of Physiology, Faculty of Medicine for Girls (Cairo), Al-Azhar University, Cairo, Egypt 2Department of Biochemistry Faculty of Pharmacy, Misr International University, Km 28, Cairo-Ismailia road, Cairo PO Box 1, Heliopolis, Cairo Egypt 3Department of Anatomy, Faculty of Medicine for Girls (Cairo), Al-Azhar University, Cairo, Egypt Mona F. Schaalan, Phone: 202/22400800, Email: [email protected] . Received 2016 Sep 28; Accepted 2016 Dec 11. Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated. This article has been cited by other articles in PMC. Diabetes is a major public health concern. In spite of continuous new drug development to treat diabetes, herbal remedies remain a potential adjunct therapy to maintain better glycemic control while also imparting few side-effects. Portulaca oleracea has been traditionally used to manage several diseases due to the anti-oxidant and anti-atherogenic effects it imparts. To better understand the mechanisms associated with potential protective effect of P. oleracea extract against diabetes, alloxan-induced diabetic rats we Continue reading >>

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