Effect Of Hyperinsulinemia And Type 2 Diabetes-like Hyperglycemia On Expressionof Hepatic Cytochrome P450 And Glutathione S-transferase Isoforms In A Newzealand Obese-derived Mouse Backcross Population.
1. J Pharmacol Exp Ther. 2002 Aug;302(2):442-50. Effect of hyperinsulinemia and type 2 diabetes-like hyperglycemia on expressionof hepatic cytochrome p450 and glutathione s-transferase isoforms in a NewZealand obese-derived mouse backcross population. Pass GJ(1), Becker W, Kluge R, Linnartz K, Plum L, Giesen K, Joost HG. (1)Institute for Pharmacology and Toxicology, Medical Faculty, Technical University of Aachen, Wendlingweg 2, D-52072 Aachen, Germany. In subgroups of a New Zealand obese mouse-derived backcross population withdefined aberrations of glucose homeostasis, a comprehensive study of the hepatic expression of cytochrome P450 and glutathione S-transferase was performed. Three patterns of alterations in response to insulin resistance(normoglycemia/hyperinsulinemia) or diabetes (hyperglycemia/hypoinsulinemia) wereobserved: mRNA levels of Cyp2b9, Cyp3a16, Cyp4a14, and Gstt2 as assessed byNorthern- and dot-blot analysis were increased markedly in liver from diabeticmice with no or only a slight increase in insulin resistant mice. Western-blotanalysis detected the corresponding changes of the CYP2B and CYP4A proteins. Incontrast, expression of Cyp2c22, Cyp2c29, and Cyp2c40 wa Continue reading >>