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Hypoglycemia Without Diabetes Diet

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Hypoglycemia (low Blood Sugar) In People Without Diabetes - Topic Overview

Hypoglycemia, or low blood sugar, is most common in people who have diabetes. If you have already been diagnosed with diabetes and need more information about low blood sugar, see the topics: You may have briefly felt the effects of low blood sugar when you've gotten really hungry or exercised hard without eating enough. This happens to nearly everyone from time to time. It's easy to correct and usually nothing to worry about. But low blood sugar, or hypoglycemia, can also be an ongoing problem. It occurs when the level of sugar in your blood drops too low to give your body energy. Ongoing problems with low blood sugar can be caused by: Medicines. Metabolic problems. Alcohol use. Symptoms can be different depending on how low your blood sugar level drops. Mild hypoglycemia can make you feel hungry or like you want to vomit. You could also feel jittery or nervous. Your heart may beat fast. You may sweat. Or your skin might turn cold and clammy. Moderate hypoglycemia often makes people feel short-tempered, nervous, afraid, or confused. Your vision may blur. You could also feel unsteady or have trouble walking. Severe hypoglycemia can cause you to pass out. You could have seizures. It Continue reading >>

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Popular Questions

  1. Qinghao Zhang

    Recently I've prepared to add insulin solution to the cell culture, but I find insulin is difficult to resolve. Below is my protocol to resolve insulin, could you give me some advise? My protocol stock solution of insulin was prepared in 0.5M Tris-HCl, pH7.0, by suspending 50mg of insulin in 2.5 mL of buffer and adjusting pH to about 3 with 1M HCl to dissolve the insulin. Then the solution was back titrated to pH 7.0 with 1M NaOH (note: the solution becomes cloudy and precipitation appears again, why ?)

  2. marie-catherine Giarratana

    Hi,
    Insulin has to be diluted at low pH for a better solubility.
    We dissolve Insulin at 1 or 2mg/mL in HCl 5mM (ie pH 2.3) but the final concentration in the culture medium is around 10µg/mL. Consequently, after a dilution factor of 100 or 200, no problem is observed for cells.

  3. Ru-Jeng Teng

    Do you mean "dissolve"? Insulin is a very water soluble protein and I can not understand why you need to adjust pH to 3.0 then titrate it back to pH 7.0. The low pH may already denature your insulin. I have never seen anybody in our lab using pH 3.0 to dissolve insulin before putting into the culture medium.

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